DOI | Trouver le DOI : https://doi.org/10.1080/03067319008026940 |
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Auteur | Rechercher : Pocklington, R.; Rechercher : Milley, J. E.1; Rechercher : Bates, S. S.1; Rechercher : Bird, C. J.1; Rechercher : De freitas, A. S. W.; Rechercher : Quilliam, M. A.1 |
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Affiliation | - Conseil national de recherches du Canada
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Format | Texte, Article |
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Sujet | domoic acid; neurotoxin; FMOC derivatization; reversed-phase HPLC; fluorescence detection; phytoplankton; Nitzschia pungens |
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Résumé | A method is presented for the trace determination of domoic acid, a neurotoxic amino acid responsible for cases of Amnesic Shellfish Poisoning resulting from the consumption of contaminated shellfish. The method involves pre-column derivatization with 9-fluorenylmethylchloroformate to form the FMOC derivative followed by reversed-phase HPLC with fluorescence detection. The detection limit for domoic acid in seawater and aqueous extracts is 15pg/mL (50 pM) using gradient elution, a 20μL injection volume, and a 2.1mm I.D. microbore column. Use of dihydrokainic acid as an internal standard improved quantitation. The method was applied to the detection of domoic acid in seawater, in phytoplankton cultures (Nitzschia pungens forma multiseries), and in natural mixed phytoplankton assemblages in estuarine waters. |
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Date de publication | 1990-03-01 |
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Maison d’édition | Taylor and Francis |
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Dans | |
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Langue | anglais |
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Publications évaluées par des pairs | Oui |
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Numéro NPARC | 23000944 |
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Exporter la notice | Exporter en format RIS |
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Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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Identificateur de l’enregistrement | f22912ef-aaf2-40ee-b581-6eb9362f3264 |
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Enregistrement créé | 2016-11-21 |
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Enregistrement modifié | 2020-03-17 |
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