| DOI | Trouver le DOI : https://doi.org/10.1385/1-59259-356-9:269 |
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| Auteur | Rechercher : Nantel, André1 |
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| Affiliation | - Conseil national de recherches du Canada. Institut de recherche en biotechnologie du CNRC
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| Format | Texte, Chapitre de livre |
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| Sujet | pharmaceutical; cancer research; protein; protein-protein interactions; mutagenesis; screening; two-hybrid; interaction trap |
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| Résumé | Acquiring functional knowledge from protein-protein interaction studies often necessitates the production of binding-impaired mutants and the study of their effects in biological systems. In many cases, multiple ligands compete for binding to the same protein domain and it becomes useful to produce specific mutations that prevent binding to one ligand but not to the others. We have combined PCR mutagenesis and a two hybrid mating assay to produce a screening strategy that has already proven useful in isolating ligand-specific mutants of the Grb10 and Raf-1 signaling proteins. |
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| Date de publication | 2003 |
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| Maison d’édition | Humana Press Inc. |
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| Dans | |
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| Série | |
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| Langue | anglais |
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| Numéro du CNRC | NRC-BRI-44804 |
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| Numéro NPARC | 3539165 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | bc13845f-8e84-4666-8bdc-a42d7fede545 |
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| Enregistrement créé | 2009-03-01 |
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| Enregistrement modifié | 2020-06-12 |
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