DOI | Trouver le DOI : https://doi.org/10.1080/02772248.2017.1338703 |
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Auteur | Rechercher : Pelletier, Guillaume; Rechercher : Padhi, Bhaja; Rechercher : Hamzeh, Mahsa1; Rechercher : Halasz, Annamaria1; Rechercher : Singh, Manjeet; Rechercher : Hawari, Jalal; Rechercher : Sunahara, Geoffrey1 |
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Affiliation | - Conseil national de recherches du Canada. Développement des cultures et des ressources aquatiques
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Format | Texte, Article |
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Sujet | in vitro assay; MDCK cells; Jatropha oil; phorbol ester; toxic equivalency factor |
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Résumé | The oil extracted from Jatropha seeds is an emerging biodiesel feedstock that also contains several pro-inflammatory phorbol esters. These phorbol esters can elicit adverse inflammatory responses through activation of the protein kinase C, as previously described for the prototypical phorbol ester 12-O-tetradecanoylphorbol-13-acetate. We extracted and purified the six phorbol esters identified in Jatropha oil and assessed their pro-inflammatory activities using a recently developed gene expression-based bioassay. Borrowing from an approach used for the assessment of dioxin toxicity, we expressed their pro-inflammatory potencies in relation to the potency of 12-O-tetradecanoylphorbol-13-acetate (in terms of 12-O-tetradecanoylphorbol-13-acetate toxic equivalency factors). The pro-inflammatory potencies of Jatropha phorbol esters were orders of magnitudes below the potency of 12-O-tetradecanoylphorbol-13-acetate. Interestingly, the cytotoxicity of phorbol esters did not appear to be directly related to their pro-inflammatory potencies. Calculations based on phorbol ester potencies and concentrations led to overestimation of the pro-inflammatory activity of Jatropha oil, as measured by the same gene expression-based bioassay. The preliminary results presented here suggest that further work on the described approach may lead to the development of valuable tools and metrics to quantify and predict the pro-inflammatory activities of complex phorbol ester mixtures. |
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Date de publication | 2017-06-06 |
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Maison d’édition | Taylor & Francis |
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Dans | |
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Langue | anglais |
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Publications évaluées par des pairs | Oui |
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Numéro NPARC | 23002169 |
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Exporter la notice | Exporter en format RIS |
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Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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Identificateur de l’enregistrement | b38a3999-eb5d-442a-bc19-92198eee053f |
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Enregistrement créé | 2017-08-29 |
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Enregistrement modifié | 2020-03-16 |
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