| Auteur | Rechercher : Rangarajan, E.; Rechercher : Bhatia, S.; Rechercher : Watson, David; Rechercher : Munger, C.; Rechercher : Cygler, Miroslaw; Rechercher : Matte, Allan; Rechercher : Young, N. |
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| Format | Texte, Article |
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| Sujet | adhesins, bacterial; bacteria; biochemistry; campylobacter; campylobacter Jejuni; Canada; chemistry; crystal; crystal-structure; crystallography, x-ray; domain; glycoprotein; glycoproteins; glycosylation; ligand binding; metabolism; n-linked; protein; protein glycosylation; protein structure, secondary; protein structure, tertiary; proteins; residues; site; state; structural; structure; surface; system |
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| Résumé | Campylobacter jejuni is unusual among bacteria in possessing a eukaryotic-like system for N-linked protein glycosylation at Asn residues in sequons of the type Asp/Glu-Xaa-Asn-Xaa-Ser/Thr. However, little is known about the structural context of the glycosylated sequons, limiting the design of novel recombinant glycoproteins. To obtain more information on sequon structure, we have determined the crystal structure of the PEB3 (Cj0289c) dimer. PEB3 has the class II periplasmic-binding protein fold, with each monomer having two domains with a ligand-binding site containing citrate located between them, and overall resembles molybdate- and sulfate-binding proteins. The sequon around Asn90 is located within a surface-exposed loop joining two structural elements. The three key residues are well exposed on the surface; hence, they may be accessible to the PglB oligosaccharyltransferase in the folded state |
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| Date de publication | 2007-05 |
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| Dans | |
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| Langue | anglais |
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| Numéro du CNRC | RANGARAJAN2007 |
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| Numéro NPARC | 3539796 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 8aea0f66-36f2-4e7c-982e-1b3c670c57eb |
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| Enregistrement créé | 2009-03-01 |
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| Enregistrement modifié | 2020-05-10 |
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