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| DOI | Trouver le DOI : https://doi.org/10.1093/femsec/fiw130 |
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| Auteur | Rechercher : Garneau, Marie-Ève; Rechercher : Michel, Christine; Rechercher : Meisterhans, Guillaume1; Rechercher : Fortin, Nathalie1; Rechercher : King, Thomas L.; Rechercher : Greer, Charles W.1; Rechercher : Lee, Kenneth |
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| Affiliation | - Conseil national de recherches du Canada. Énergie, les mines et l'environnement
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| Format | Texte, Article |
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| Sujet | arctic; sea ice; hydrocarbon biodegradation; bioremediation; 16S ribosomal RNA gene sequencing; microbial community fingerprinting |
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| Résumé | The increasing accessibility to navigation and offshore oil exploration brings risks of hydrocarbon releases in Arctic waters. Bioremediation of hydrocarbons is a promising mitigation strategy but challenges remain, particularly due to low microbial metabolic rates in cold, ice-covered seas. Hydrocarbon degradation potential of ice-associated microbes collected from the Northwest Passage was investigated. Microcosm incubations were run for 15 days at -1.7°C with and without oil to determine the effects of hydrocarbon exposure on microbial abundance, diversity and activity, and to estimate component-specific hydrocarbon loss. Diversity was assessed with automated ribosomal intergenic spacer analysis and ion torrent 16S rRNA gene sequencing. Bacterial activity was measured by ³H-leucine uptake rates. After incubation, sub-ice and sea-ice communities degraded 94% and 48% of the initial hydrocarbons, respectively. Hydrocarbon exposure changed the composition of sea-ice and sub-ice communities; in sea-ice microcosms, Bacteroidetes (mainly Polaribacter) dominated whereas in sub-ice microcosms, Epsilonproteobacteria contribution increased, but that of Alphaproteobacteria and Bacteroidetes decreased. Sequencing data revealed a decline in diversity and increases in Colwellia and Moritella in oil-treated microcosms. Low concentration of dissolved organic matter (DOM) in sub-ice seawater may explain higher hydrocarbon degradation when compared to sea ice, where DOM was abundant and composed of labile exopolysaccharides. |
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| Date de publication | 2016-07-06 |
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| Maison d’édition | Oxford University Press
Federation of European Microbiological Societies |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 23000460 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 7c0aa52f-edde-4536-bc0b-f3df34a692ee |
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| Enregistrement créé | 2016-07-20 |
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| Enregistrement modifié | 2024-02-05 |
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