DOI | Trouver le DOI : https://doi.org/10.1166/jns.2009.1007 |
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Auteur | Rechercher : Pierson, Laure-Anne; Rechercher : Lalanne, Muriel; Rechercher : Brambilla, Davide; Rechercher : Marsaud, Véronique; Rechercher : Nicolas, Valérie; Rechercher : Ball, Rita; Rechercher : Stanimirovic, Danica1; Rechercher : Couvreur, Patrick; Rechercher : Andrieux, Karine |
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Affiliation | - Conseil national de recherches du Canada
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Format | Texte, Article |
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Sujet | brain endothelial cells; didanosine; glycerolipidic prodrug; hiv; PEGylated nanoparticles |
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Résumé | PEGylated biodegradable nanoparticles based on poly(alkyl cyanoacrylate) polymers have been investigated to encapsulate didanosine prodrugs as an approach to treat HIV-1 associated dementia. To achieve this goal, an amphiphilic poly[(poly(ethylene glycol) cyanoacrylate)-co-(hexadecyl cyanoacrylate)] (PEGCA-PHDCA) copolymer has been synthesized and two different didanosine prodrugs have been used; the dipalmitoyl glycerol didanosine (ProddINP) and the dipalmitoyl glycerol phosphate didanosine (ProddIP), only differ by the presence or not of a phosphate group in their chemical structure. By means of differential scanning calorimetry (DSC), the capacity of PEGCA-PHDCA copolymer to incorporate ProddINP and ProddIP prodrugs was investigated. PEGCA-PHDCA nanoparticles were then prepared via an optimised solvent emulsion/evaporation technique using the incorporation ratios determined by DSC. Finally, confocal laser scanning microscopy experiments have shown these prodrugs-loaded nanoparticles were uptaken by rat immortalised brain endothelial cells (SV-ARBEC). These first results appeared promising for further in vitro and in vivo evaluation of these nanoparticles. |
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Date de publication | 2009-12 |
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Dans | |
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Langue | anglais |
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Publications évaluées par des pairs | Oui |
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Numéro NPARC | 21275165 |
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Exporter la notice | Exporter en format RIS |
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Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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Identificateur de l’enregistrement | 30327620-6fc6-4b8e-8ef5-50dba99bb491 |
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Enregistrement créé | 2015-05-21 |
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Enregistrement modifié | 2020-04-16 |
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