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Comparison of the biochemical composition of normal epidermal mucus extruded slime of hagfish (Myxine glutinosa L.)

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1016/j.fsi.2008.08.012
AuteurRechercher : ; Rechercher : 1; Rechercher : 2
Affiliation
  1. Conseil national de recherches du Canada. Programme d'aide à la recherche industrielle
  2. Conseil national de recherches du Canada. Développement des cultures et des ressources aquatiques
FormatTexte, Article
Résumé
Hagfish (Myxine glutinosa) secrete normal epidermal mucus and extruded slime. The epidermal mucus is produced continuously to prevent pathogen adherence while the extruded slime is observed predominantly during feeding, provocation or stress. To date little is known about the involvement of extruded slime in the physiological functions of hagfish. In this preliminary study, innate immune enzymes and the protein composition of hagfish normal epidermal mucus and extruded slime were analysed and compared. The lysozyme specific activity of hagfish was observed approximately two-fold higher in extruded slime than that of epidermal mucus. The extruded slime had approximately 3.5–5.0 fold increased levels of alkaline phosphatase, cathepsin B and proteases in comparison to epidermal mucus. Protease characterization using specific inhibitors showed that the extruded slime had higher levels of serine trypsin-like proteases compared to metalloproteases whereas epidermal mucus showed equal proportion of both serine and metalloproteases. SDS-PAGE analysis showed high levels of three proteins with molecular masses in the range of 13–16 kDa in the extruded slime. The LC/MS/MS analysis of protein bands 1, 2 and 3 showed closest matches to hemoglobulin-3, histone H3 and H2B proteins, respectively. The observation of elevated levels of innate immune parameters in the extruded slime suggested that the extruded slime has a significant role in innate immunity of hagfish against infectious pathogens
Date de publication
Dans
  • Fish & Shellfish Immunology 25, nº 5 (novembre 2008) : 625–632.
Langueanglais
Publications évaluées par des pairsOui
Numéro du CNRCNRCC 42788
1751
Numéro NPARC3538373
Exporter la noticeExporter en format RIS
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Identificateur de l’enregistrement0122a053-b300-4e59-9083-db46645ac024
Enregistrement créé2009-03-01
Enregistrement modifié2020-04-15
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