Comparative assessment of next-generation sequencing, denaturing gradient gel electrophoresis, clonal restriction fragment length polymorphism and cloning-sequencing as methods for characterizing commercial microbial consortia

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DOIResolve DOI: http://doi.org/10.1016/j.mimet.2014.11.013
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TypeArticle
Journal titleJournal of Microbiological Methods
ISSN0167-7012
Volume108
Pages103111; # of pages: 9
SubjectDNA 16S; hydrocarbon; diagnostic kit; Arcobacter; bacterium detection; controlled study; denaturing gradient gel electrophoresis; DNA sequence; entomopathogenic bacterium; environmental health; gene amplification; intermethod comparison; microbial community; microbial consortium; molecular cloning; polymerase chain reaction; restriction fragment length polymorphism; risk assessment; sequence analysis; soil pollution; water contamination; amplified fragment length polymorphism; bacterium; bioreactor; comparative study; denaturing gradient gel electrophoresis; diagnostic kit; high throughput sequencing; isolation and purification; restriction fragment length polymorphism; Arcobacter; Bacteria (microorganisms); Amplified Fragment Length Polymorphism Analysis; Bacteria; Bioreactors; Denaturing Gradient Gel Electrophoresis; High-Throughput Nucleotide Sequencing; Microbial Consortia; Polymorphism, Restriction Fragment Length; Reagent Kits, Diagnostic; Sequence Analysis, DNA
Abstract
Publication date
PublisherElsevier
LanguageEnglish
AffiliationNational Research Council Canada; Human Health Therapeutics
Peer reviewedYes
NPARC number21276961
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Record identifierb3c31b02-5bce-4104-bad0-5d8233f0cd2b
Record created2015-11-10
Record modified2016-05-09
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