| Auteur | Rechercher : Abulrob, A.1; Rechercher : Lu, Z.2; Rechercher : Brunette, E.1; Rechercher : Pulla, D.2; Rechercher : Stanimirovic, D.1; Rechercher : Johnston, L. J.2 |
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| Affiliation | - Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
- Conseil national de recherches du Canada. Institut Steacie des sciences moléculaires du CNRC
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| Format | Texte, Article |
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| Sujet | confocal microscopy; glycolipid; membrane rafts; near-field scanning optical microscopy |
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| Résumé | The localization of asialo-GM1 in ordered membrane raft domains in HeLa cells has been examined using a combination of membrane fractionation and fluorescence imaging. The glycolipid is enriched in Triton X-100 insoluble membrane fractions that contain high concentrations of cholesterol and caveolin-1 but is also found in detergent soluble membrane fractions. Near-field fluorescence microscopy shows that a fraction of the asialo-GM1 is localized in small nanoscale clusters that have an upper limit for the average diameter of approximately 90 nm and are partially colocalized with caveolae membrane domains. In addition to clusters, a diffuse, non-clustered population of asialo-GM1 is observed and is hypothesized to correspond to glycolipid isolated in detergent soluble membrane fractions. |
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| Date de publication | 2008 |
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| Dans | |
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| Langue | anglais |
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| Numéro du CNRC | ABULROB2008B |
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| Numéro NPARC | 9381631 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | ffee2abb-a5ea-4847-a530-083d118fb406 |
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| Enregistrement créé | 2009-07-10 |
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| Enregistrement modifié | 2020-04-15 |
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