| Téléchargement | - Voir le manuscrit accepté : Development of patch-clamp chips for mammalian cell applications (PDF, 937 Kio)
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| Auteur | Rechercher : Martinez, Dolores1; Rechercher : Martina, Marzia2; Rechercher : Kremer, Laura1; Rechercher : Monette, Robert2; Rechercher : Comas, Tanya2; Rechercher : Salim, Danish2; Rechercher : Py, Christophe1; Rechercher : Denhoff, Mike W.1; Rechercher : Mealing, Geoff2 |
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| Affiliation | - Conseil national de recherches du Canada. Institut des sciences des microstructures du CNRC
- Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
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| Format | Texte, Article |
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| Sujet | Mammalian cells; Planar patch-clamp; Neurochip; Whole-cell recordings |
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| Résumé | We have previously described the designs of two planar patch-clamp neurochips and their application to the electrophysiological study of molluscan neurons cultured on-chip. Neuron attachment and growth over apertures on the neurochip surface permitted the acquisition of whole-cell patch-clamp recordings. To broaden the application of these neurochips from molluscan to mammalian neurons, we conducted a study of cell-to-aperture interaction to optimize conditions for these smaller, more fragile cells. For this purpose, we designed a “sieve” chip having multiple apertures on its surface. Random growth of rat cortical neurons resulted in a 32% (n = 324) probability of cell growth over 2 μm diameter apertures; larger diameters resulted in growth through the aperture. Based on these findings, single-aperture neurochips were fabricated having 2 μm diameter aperture and preliminary electrophysiological recordings from cortical cultures at 14 DIV are presented. The implications of this study for the next-generation neurochips are discussed. |
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| Date de publication | 2010-11-28 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 17175248 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | fed3f356-458d-4def-88b1-0531e5c6d26b |
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| Enregistrement créé | 2011-03-26 |
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| Enregistrement modifié | 2020-04-17 |
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