DOI | Trouver le DOI : https://doi.org/10.1073/pnas.1214809110 |
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Auteur | Rechercher : Kuss, S.; Rechercher : Polcari, D.; Rechercher : Geissler, M.1; Rechercher : Brassard, D.1; Rechercher : Mauzeroll, J. |
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Affiliation | - Conseil national de recherches du Canada
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Format | Texte, Article |
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Sujet | ferrocene derivative; ferrocenemethanol; multidrug resistance protein 1; ruthenium complex; unclassified drug; cancer cell; cell culture; cell structure; electrochemistry; female; HeLa cell; human cell; kinetics; multidrug resistance; protein localization; quantitative analysis; scanning electrochemical microscopy; target cell; uterine cervix cancer; HeLa cells; microelectrode; MRP1; Cell Culture Techniques; Drug Resistance, Multiple; Female; Ferrous Compounds; HeLa Cells; Microelectrodes; Microscopy, Fluorescence; Microscopy, Scanning Probe; Multidrug Resistance-Associated Proteins |
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Résumé | The emergence of resistance to multiple unrelated chemotherapeutic drugs impedes the treatment of several cancers. Although the involvement of ATP-binding cassette transporters has long been known, there is no in situ method capable of tracking this transporter- related resistance at the single-cell level without interfering with the cell's environment or metabolism. Here, we demonstrate that scanning electrochemical microscopy (SECM) can quantitatively and noninvasively track multidrug resistance-related protein 1- dependent multidrug resistance in patterned adenocarcinoma cervical cancer cells. Nonresistant human cancer cells and their multidrug resistant variants are arranged in a side-by-side format using a stencil-based patterning scheme, allowing for precise positioning of target cells underneath the SECM sensor. SECM measurements of the patterned cells, performed with ferrocenemethanol and [Ru(NH3)6]3+ serving as electrochemical indicators, are used to establish a kinetic "map" of constant-height SECM scans, free of topography contributions. The concept underlying the work described herein may help evaluate the effectiveness of treatment administration strategies targeting reduced drug efflux. |
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Date de publication | 2013 |
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Dans | |
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Langue | anglais |
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Publications évaluées par des pairs | Oui |
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Numéro NPARC | 21270567 |
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Exporter la notice | Exporter en format RIS |
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Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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Identificateur de l’enregistrement | fcc2b639-e891-4e35-bed1-4f7e76890995 |
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Enregistrement créé | 2014-02-17 |
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Enregistrement modifié | 2020-04-22 |
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