| Téléchargement | - Voir le manuscrit accepté : Introducing a new bioengineered bug: Methylobacterium extorquens tuned as a microbial bioplastic factory (PDF, 1.3 Mio)
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| DOI | Trouver le DOI : https://doi.org/10.4161/bbug.2.2.15009 |
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| Auteur | Rechercher : Höfer, Philipp; Rechercher : Vermette, Patrick; Rechercher : Groleau, Denis1 |
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| Affiliation | - Conseil national de recherches du Canada. Institut de recherche en biotechnologie du CNRC
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| Format | Texte, Article |
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| Sujet | Acids; activity; Acyl-CoA desaturase; Bacteria; biotechnology; biosynthesis; Biotechnology; Canada; Carbon; Enzymes; Escherichia coli; Fatty Acids; Functionalized polyhydroxyalkanoates; Funtionalized polyhydroxyalkanoates; Genes; Genetic Engineering; genome; Genome sequence; Hydrocarbons; Hydrolysis; Isomerase-dependent beta-oxidation; Liver; metabolism; Methane; methane oxidation; Methanol; Methylobacterium extorquens; microbiology; Mitochondria; Mutagenesis; plastic; Plastics; Protein; Purification; rat; Reductase-dependent beta-oxidation; Scopus; Temperature; Unsaturated fatty acids; Yeast |
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| Résumé | Discussion on and use of methanol as chemical feedstock and as alternative fuel has gained momentum during the past years. Consequently, microorganism and product design based on "methylotrophism" is in vogue as reflected by increasing research and development activities in methanolrelated areas. A recent example of microorganism and product development is the use of recombinant Methylobacterium extorquens ATCC 55366 strains in the production of second generation biopolyesters. Feeding n-alkenoic acids in addition to methanol yielded functionalized polyhydroxyalkanoates (PHAs) and uncovered how M. extorquens copes with fatty acids. While some parts of the degradation pathway remain unclear, possible metabolic routes are suggested that may explain the significant loss of double bonds prior to polymerization of PHA precursors and occurrence of oddnumbered monomers derived from evennumbered n-alkenoic acids. In addition, microbial discoloration upon fatty acid feeding is discussed and future directions for further genetic modification of M. extorquens are provided. -¬ 2011 Landes |
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| Date de publication | 2011-01-01 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | NRCC 52781 |
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| Numéro NPARC | 18335884 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | edbf619e-4a8c-41e7-bad0-51bbf04a78a1 |
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| Enregistrement créé | 2011-07-28 |
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| Enregistrement modifié | 2022-11-18 |
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