| DOI | Trouver le DOI : https://doi.org/10.1093/neuonc/nor183 |
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| Auteur | Rechercher : Tomanek, B.1; Rechercher : Iqbal, U.2; Rechercher : Blasiak, B.1; Rechercher : Abulrob, A.2; Rechercher : Albaghdadi, H.2; Rechercher : Matyas, J.R.; Rechercher : Ponjevic, D.; Rechercher : Sutherland, G.R. |
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| Affiliation | - Conseil national de recherches du Canada. Institut du biodiagnostic du CNRC
- Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
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| Format | Texte, Article |
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| Sujet | binding protein; contrast medium; dextran; growth factor; nuclear magnetic resonance imaging agent; superparamagnetic iron oxide; vascular targeting agent; animal experiment; animal model; animal tissue; brain blood vessel; brain tumor; cancer tissue; fluorescence microscopy; glioblastoma; in vivo study; infrared radiation; inoculation; male; mouse; near infrared spectroscopy; nuclear magnetic resonance imaging; relaxation time; Antibodies; Brain Neoplasms; Cell Line, Tumor; Contrast Media; Dextrans; Ferric Compounds; Glioblastoma; Insulin-Like Growth Factor Binding Proteins; Magnetic Resonance Imaging; Male; Mice; Mice, Nude; Microscopy, Fluorescence; Nanoparticles |
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| Résumé | A mouse model of glioblastoma multiforme was used to determine the accumulation of a targeted contrast agent in tumor vessels. The contrast agent, consisting of superparamagnetic iron oxide coated with dextran, was functionalized with an anti-insulin-like-growth-factor binding protein 7 (anti-IGFBP7) single domain antibody. The near infrared marker, Cy5.5, was also attached for an in vivo fluorescence study. A 9.4T magnetic resonance imaging (MRI) system was used for in vivo studies on days 10 and 11 following tumor inoculation. T2 relaxation time was used to measure the accumulation of the contrast agent in the tumor. Changes in tumor to brain contrast because of active targeting were compared with a nontargeted contrast agent. Effective targeting was confirmed with near infrared measurements and fluorescent microscopic analysis. The results showed that there was a statistically significant (P < .01) difference in normalized T2 between healthy brain and tumor tissue 10 min, 1 h, and 2 h point postinjection of the anti-IGFBP7 single domain antibody targeted and nontargeted iron oxide nanoparticles. A statistical difference remained in animals treated with targeted nanoparticles 24 h postinjection only. The MRI, near infrared imaging, and fluorescent microscopy studies showed corresponding spatial and temporal changes. We concluded that the developed anti-IGFBP7-iron oxide single domain antibodytargeted MRI contrast agent selectively binds to abnormal vessels within a glioblastoma. T2-weighted MRI and near infrared imaging are able to detect the targeting effects in brain tumors. © The Author(s) 2011. |
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| Date de publication | 2012 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 21269457 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | e53e0f53-9eaf-4f78-8e84-5b7a98eff04d |
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| Enregistrement créé | 2013-12-12 |
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| Enregistrement modifié | 2020-04-21 |
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