| Téléchargement | - Voir le manuscrit accepté : Cell placement and guidance on substrates for neurochip interfaces (PDF, 735 Kio)
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| DOI | Trouver le DOI : https://doi.org/10.1002/bit.22539 |
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| Auteur | Rechercher : Charrier, Anne1; Rechercher : Martinez, Dolores2; Rechercher : Monette, Robert3; Rechercher : Comas, Tanya3; Rechercher : Movileanu, Raluca2; Rechercher : Py, Christophe2; Rechercher : Denhoff, Mike2; Rechercher : Krantis, Anthony; Rechercher : Mealing, Geoff3 |
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| Affiliation | - Conseil national de recherches du Canada. Institut Steacie des sciences moléculaires du CNRC
- Conseil national de recherches du Canada. Institut des sciences des microstructures du CNRC
- Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
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| Format | Texte, Article |
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| ISSN | 1097-0290 |
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| Sujet | neurochip interface; cell placement; neurites guidance; proteins; patterning; PDMS stamp |
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| Résumé | Interface devices such as integrated planar patch-clamp chips are being developed to study the electrophysiological activity of neuronal networks grown in vitro. The utility of such devices will be dependent upon the ability to align neurons with interface features on the chip by controlling neuronal placement and by guiding cell connectivity. In this paper, we present a strategy to accomplish this goal. Patterned chemical modification of SiN surfaces with poly-D-lysine transferred from PDMS stamps was used to promote adhesion and guidance of cryo-preserved primary rat cortical neurons. We demonstrate that these neurons can be positioned and grown over microhole features which will ultimately serve as patch-clamp interfaces on the chip. |
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| Date de publication | 2009 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Non |
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| Numéro NPARC | 15545426 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | db293500-bf12-47dd-b91c-4ce48b7788ce |
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| Enregistrement créé | 2010-08-16 |
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| Enregistrement modifié | 2020-04-16 |
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