| DOI | Trouver le DOI : https://doi.org/10.1042/BJ20081131 |
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| Auteur | Rechercher : Luga, Valbona; Rechercher : Mclean, Sarah; Rechercher : Le Roy, Christine; Rechercher : O'Connor-McCourt, Maureen1; Rechercher : Wrana, Jeffrey L.; Rechercher : Di Guglielmo, Gianni M. |
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| Affiliation | - Conseil national de recherches du Canada. Institut de recherche en biotechnologie du CNRC
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| Format | Texte, Article |
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| Sujet | granulocyte/macrophage colony-stimulating factor (GMCSF); mannosyl(α-1,6)-glycoprotein β-1,6-N-acetylglucosaminyltransferase V (Mgat5); transforming growth factor β (TGFβ); transforming growth factor β type II receptor (TβRII) |
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| Résumé | Cell-surface TGFβ (transforming growth factor β) receptors partition into membrane rafts and the caveolin-positive endocytic compartment by an unknown mechanism. In the present study, we investigated the determinant in the TGFβ type II receptor (TβRII) that is necessary for membrane raft/caveolar targeting. Using subcellular fractionation and immunofluorescence microscopy techniques, we demonstrated that the extracellular domain of TβRII mediates receptor partitioning into raft and caveolin-positive membrane domains. Pharmacological perturbation of glycosylation using tunicamycin or the mutation of Mgat5 [mannosyl(α-1,6)-glycoprotein β-1,6-N-acetylglucosaminyltransferase V] activity interfered with the raft partitioning of TβRII. However, this was not due to the glycosylation state of TβRII, as a non-glycosylated TβRII mutant remained enriched in membrane rafts. This suggested that other cell-surface glycoproteins associate with the extracellular domain of TβRII and direct their partitioning in membrane raft domains. To test this we analysed a GMCSF (granulocyte/macrophage colony-stimulating factor)–TβRII chimaeric receptor, which contains a glycosylated GMCSF extracellular domain fused to the transmembrane and intracellular domains of TβRII. This chimaeric receptor was found to be largely excluded from membrane rafts and caveolin-positive structures. Our results indicate that the extracellular domain of TβRII mediates receptor partitioning into membrane rafts and efficient entrance into caveolin-positive endosomes. |
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| Date de publication | 2009-04-08 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | NRCC 53197 |
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| Numéro NPARC | 21268265 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | cdad4fca-05d9-4f35-9dc7-10db2bb46621 |
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| Enregistrement créé | 2013-06-11 |
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| Enregistrement modifié | 2020-04-16 |
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