DOI | Trouver le DOI : https://doi.org/10.1093/glycob/cws071 |
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Auteur | Rechercher : Schur, M.J.1; Rechercher : Lameignere, E.; Rechercher : Strynadka, N.C.J.; Rechercher : Wakarchuk, W.W.1 |
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Affiliation | - Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
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Format | Texte, Article |
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Sujet | alpha 2,3 sialyltransferase; alpha 2,6 sialyltransferase; galactose; sialyltransferase; unclassified drug; aminosugar; bacterial protein; beta D galactoside alpha 2 6 sialyltransferase; beta galactoside alpha 2,3 sialyltransferase; beta-D-galactoside alpha 2-6-sialyltransferase; beta-galactoside alpha-2,3-sialyltransferase; hybrid protein; magnesium; manganese; n acetyllactosamine; N-acetyllactosamine; sialic acid derivative; sialyltransferase; bacterial genome; bacterium isolate; cheetah; enzyme activation; enzyme analysis; enzyme substrate; Escherichia coli; gene expression; gene sequence; Helicobacter; Helicobacter acinonychis; molecular cloning; nucleotide sequence; protein expression; sequence homology; tandem repeat; biosynthesis; chemical structure; chemistry; conformation; enzyme active site; enzyme specificity; enzymology; genetics; glycosylation; pH; structural homology; Escherichia coli; Helicobacter; Helicobacter acinonychis; Amino Sugars; Bacterial Proteins; Carbohydrate Conformation; Catalytic Domain; Cloning, Molecular; Glycosylation; Helicobacter; Hydrogen-Ion Concentration; Magnesium; Manganese; Models, Molecular; Recombinant Fusion Proteins; Sialic Acids; Sialyltransferases; Structural Homology, Protein; Substrate Specificity |
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Résumé | Genome sequence data were used to clone and express two sialyltransferase enzymes of the GT-42 family from Helicobacter acinonychis ATCC 51104, a gastric disease isolate from Cheetahs. The deposited genome sequence for these genes contains a large number of tandem repeat sequences in each of them: HAC1267 (RQKELE)15 and HAC1268 (EEKLLEFKNI)13. We obtained two clones with different numbers of repeat sequences for the HAC1267 gene homolog and a single clone for the HAC1268 gene homolog. Both genes could be expressed in Escherichia coli and sialyltransferase activity was measured using synthetic acceptor substrates containing a variety of terminal sugars. Both enzymes were shown to have a preference for N-acetyllactosamine, and they each made a product with a different linkage to the terminal galactose. HAC1267 is a mono-functional 2,3-sialyltransferase, whereas HAC1268 is a mono-functional α2,6- sialyltransferase and is the first member of GT-42 to show α2,6- sialyltransferase activity. © 2012 The Author. |
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Date de publication | 2012 |
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Dans | |
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Langue | anglais |
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Publications évaluées par des pairs | Oui |
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Numéro NPARC | 21269286 |
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Exporter la notice | Exporter en format RIS |
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Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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Identificateur de l’enregistrement | cc4e5c5d-dbaa-4045-88e7-abd3b5b11f35 |
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Enregistrement créé | 2013-12-12 |
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Enregistrement modifié | 2022-11-18 |
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