| Téléchargement | - Voir la version finale : Pentavalent single-domain antibodies reduce Campylobacter jejuni motility and colonization in chickens (PDF, 1.4 Mio)
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| DOI | Trouver le DOI : https://doi.org/10.1371/journal.pone.0083928 |
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| Auteur | Rechercher : Riazi, Ali; Rechercher : Strong, Philippa C. R.1; Rechercher : Coleman, Russell1; Rechercher : Chen, Wangxue1; Rechercher : Hirama, Tomoko2; Rechercher : van Faassen, Henk1; Rechercher : Henry, Matthew; Rechercher : Logan, Susan M.1; Rechercher : Szymanski, Christine M.; Rechercher : MacKenzie, Roger1; Rechercher : Ghahroudi, Mehdi Arbabi1 |
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| Affiliation | - Conseil national de recherches du Canada. Thérapeutique en santé humaine
- Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
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| Format | Texte, Article |
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| Résumé | Campylobacter jejuni is the leading cause of bacterial foodborne illness in the world, with symptoms ranging from acute diarrhea to severe neurological disorders. Contaminated poultry meat is a major source of C. jejuni infection, and therefore, strategies to reduce this organism in poultry, are expected to reduce the incidence of Campylobacter-associated diseases. We have investigated whether oral administration of C. jejuni-specific single-domain antibodies would reduce bacterial colonization levels in chickens. Llama single-domain antibodies specific for C. jejuni were isolated from a phage display library generated from the heavy chain IgG variable domain repertoire of a llama immunized with C. jejuni flagella. Two flagella-specific single-domain antibodies were pentamerized to yield high avidity antibodies capable of multivalent binding to the target antigen. When administered orally to C. jejuni-infected two-day old chicks, the pentabodies significantly reduced C. jejuni colonization in the ceca. In vitro, the motility of the bacteria was also reduced in the presence of the flagella-specific pentabodies, suggesting the mechanism of action is through either direct interference with flagellar motility or antibody-mediated aggregation. Fluorescent microscopy and Western blot analyses revealed specific binding of the anti-flagella pentabodies to the C. jejuni flagellin. |
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| Date de publication | 2013-12-31 |
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| Maison d’édition | PLOS |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 23000585 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | c9f638d2-25f9-438f-b2d9-52b5cfa4d3a6 |
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| Enregistrement créé | 2016-08-03 |
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| Enregistrement modifié | 2021-09-20 |
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