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Reinvestigation of the structure of Brucella O-antigens

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1016/j.carres.2013.03.021
AuteurRechercher : ; Rechercher : 1
Affiliation
  1. Conseil national de recherches du Canada
FormatTexte, Article
Sujet2-D NMR; Brucella; LPS; MS; O-Chain; O-specific polysaccharides; Pentasaccharides; Tetrasaccharides; Nuclear magnetic resonance; Polymers; Strain; Structure (composition); Epitopes; epitope; O antigen; pentasaccharide; tetrasaccharide; acetylation; antigen structure; Brucella melitensis; carbon nuclear magnetic resonance; deacylation; proton nuclear magnetic resonance; Brucella; Brucella melitensis; Brucella melitensis 16M
Résumé
O-Specific polysaccharides of Brucella contain two antigenic determinants, called A and M. Most of the strains express epitope A with a small amount of epitope M, whereas Brucella melitensis strain 16 M expresses longer polymer consisting mostly of M-type epitopes. Proposed explanation was that epitope A is defined by 1-2-linked homopolymer of N-formylperosamine (Rha4NFo), while epitope M is a pentasaccharide with four 2- and one 3-substituted Rha4NFo. We reinvestigated both types of structures by 2D NMR and showed that M-epitope is a tetrasaccharide, missing one of the 2-linked Rha4NFo as compared to the previously proposed structure. Polysaccharide from B. melitensis 16 M contains a fragment of 1-2-linked polymer, capped with M-type polymer. Other strains contain one or two M-type units at the non-reducing end of the 1-2-linked O-chain. Crown Copyright © 2013 Published by Elsevier Ltd.
Date de publication
Dans
  • Carbohydrate Research 378 (2013) : 144–147.
Langueanglais
Publications évaluées par des pairsOui
Numéro NPARC21270650
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Identificateur de l’enregistrementc6f9a02c-a976-4c8a-a40a-f32fb9bd0dc1
Enregistrement créé2014-02-17
Enregistrement modifié2020-04-22
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