| DOI | Trouver le DOI : https://doi.org/10.1364/BOE.4.001051 |
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| Auteur | Rechercher : Watts, B.R.; Rechercher : Zhang, Z.1; Rechercher : Xu, C.-Q.; Rechercher : Cao, X.; Rechercher : Lin, M. |
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| Affiliation | - Conseil national de recherches du Canada
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| Format | Texte, Article |
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| Sujet | Design modifications; False positive rates; Forward scatters; Integrated device; Integrated microfluidic devices; Optimal excitation; Polystyrene beads; Side scatter signal; Geometry; Photoexcitation; Polystyrenes; Signal detection; device fabrication; device testing; experimental design; fluorescence imaging; free space detection; laboratory diagnosis; light scattering; limit of detection; microfluidic analysis; optical excitation; optics; scatter detection |
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| Résumé | A photonic integrated microfluidic device is demonstrated to perform optical excitation and forward scatter collection all on-chip in a planar format. Integrated on-chip optics formed a tailored beam geometry for optimal excitation of particles while a special design modification allowed for on-chip forward collection with the beam shaping capabilities. A notch was placed in the lens system that caused a dark spot on the facet of a collection waveguide while not affecting the beam geometry at the point of interrogation. The modified device with the ability to form a 10 μm beam geometry was demonstrated to detect the forward scatter from blank 5 μm diameter polystyrene beads. Free-space collection of side scatter signals was performed simultaneously with the on-chip collection and the designs demonstrated and enhanced SNR while the reliability of detection was determined to be appropriate for many applications. Excellent performance was confirmed via a false positive rate of 0.4%, a missed events rate of 6.8%, and a coincident rate of 96.3% as determined between simultaneously performed free-space and on-chip detection schemes. © 2013 Optical Society of America. |
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| Date de publication | 2013 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 21270547 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | b60201b1-f4c3-46bb-a8e9-c184a1b35768 |
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| Enregistrement créé | 2014-02-17 |
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| Enregistrement modifié | 2020-04-22 |
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