| Téléchargement | - Voir le manuscrit accepté : Derivatives of thiocolchicine and its applications to CEM cells treatment using 19F Magnetic Resonance ex vivo (PDF, 698 Kio)
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| DOI | Trouver le DOI : https://doi.org/10.1016/j.bioorg.2009.10.002 |
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| Auteur | Rechercher : Bartusik, Dorota1; Rechercher : Tomanek, Boguslaw1; Rechercher : Lattova, Erika; Rechercher : Perreault, Hélène; Rechercher : Tuszynski, Jack; Rechercher : Fallone, Gino |
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| Affiliation | - Conseil national de recherches du Canada. Institut du biodiagnostic du CNRC
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| Format | Texte, Article |
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| Sujet | Thiocolchicine; Human T-Lymphoblastoid; Three-dimensional cell culture; Magnetic Resonance Imaging; High Performance Liquid Chromatography coupled with Ultra Violet |
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| Résumé | It was shown, that cultured ex vivo human T-Lymphoblastoid (CEM) cells respond to synthesized thiocolchicine and fluorine thiocolchicine derivatives. The preparation of derivatives with substitution at C-3 and C-7 is described. All compounds were used at concentration from 1 nM to 1000 nM. Inhibitory effects of these compounds were examined in the three-dimensional (3-D) culture and cells morphology during treatment was monitored using 9.4 T MRI system. We performed studies of these compounds in CEM cells ex vivo using 1H and 19F Magnetic Resonance Imaging (MRI), 19F Magnetic Resonance Spectroscopy (MRS), High Performance Liquid Chromatography coupled with Ultra Violet (HPLC-UV) and Electron Impact Mass Spectrometry (EIMS). The results of the multi-technique approach are consistent with the fact that the new derivatives are more efficient than colchicine and thiocolchicine ex vivo. |
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| Date de publication | 2010-02-01 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 17653081 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 9601f55c-c537-459c-bc67-6ee170a072bf |
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| Enregistrement créé | 2011-04-03 |
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| Enregistrement modifié | 2020-04-17 |
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