Broad-spectrum biofilm inhibition by Kingella kingae exopolysaccharide

Par Conseil national de recherches du Canada

DOI	Trouver le DOI : https://doi.org/10.1128/JB.00311-11
Auteur	Rechercher : Bendaoud, M.; Rechercher : Vinogradov, E.¹; Rechercher : Balashova, N.V.; Rechercher : Kadouri, D.E.; Rechercher : Kachlany, S.C.; Rechercher : Kaplan, J.B.
Affiliation	Conseil national de recherches du Canada
Format	Texte, Article
Sujet	exopolysaccharide; galactan; galactofuranosyl transferase; mutase; transferase; UDP galactopyranose mutase; unclassified drug; Actinobacillus pleuropneumoniae; Aggregatibacter actinomycetemcomitans; biofilm; Candida albicans; carbohydrate analysis; carbohydrate synthesis; cell surface; chemical analysis; Escherichia coli; gene cluster; inhibition kinetics; Kingella kingae; Klebsiella pneumoniae; Staphylococcus aureus; Staphylococcus epidermidis; Bacterial Physiological Phenomena; Bacterial Proteins; Biofilms; Down-Regulation; Fungi; Kingella kingae; Molecular Sequence Data; Polysaccharides, Bacterial; Actinobacillus pleuropneumoniae serovar 5; Bacteria (microorganisms); Candida albicans; Escherichia coli; Kingella kingae; Klebsiella pneumoniae; Staphylococcus aureus; Staphylococcus epidermidis
Résumé	Cell-free extracts prepared from Kingella kingae colony biofilms were found to inhibit biofilm formation by Aggregatibacter actinomycetemcomitans, Klebsiella pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Candida albicans, and K. kingae. The extracts evidently inhibited biofilm formation by modifying the physicochemical properties of the cell surface, the biofilm matrix, and the substrate. Chemical and biochemical analyses indicated that the biofilm inhibition activity in the K. kingae extract was due to polysaccharide. Structural analyses showed that the extract contained two major polysaccharides. One was a linear polysaccharide with the structure →6)-α-D-GlcNAcp-(1→5)-β-D-OclAp-(2→, which was identical to a capsular polysaccharide produced by Actinobacillus pleuropneumoniae serotype 5. The second was a novel linear polysaccharide, designated PAM galactan, with the structure →3)-β-D-Galf-(1→6)-β-D-Galf-(1→. Purified PAM galactan exhibited broad-spectrum biofilm inhibition activity. A cluster of three K. kingae genes encoding UDP-galactopyranose mutase (ugm) and two putative galactofuranosyl transferases was sufficient for the synthesis of PAM galactan in Escherichia coli. PAM galactan is one of a growing number of bacterial polysaccharides that exhibit antibiofilm activity. The biological roles and potential technological applications of these molecules remain unknown. © 2011, American Society for Microbiology.
Date de publication	2011
Dans	Journal of Bacteriology 193, nº 15 (2011) : 3879–3886.
Langue	anglais
Publications évaluées par des pairs	Oui
Numéro NPARC	21271628
Exporter la notice	Exporter en format RIS
Signaler une correction	Signaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement	93f29cca-f8a9-4c7d-986f-76739a56e66c
Enregistrement créé	2014-03-24
Enregistrement modifié	2022-11-18

Date de modification :: 2024-04-20