Identification of a sialate-O-acetyltransferase from Campylobacter jejuni: demonstration of direct transfer to the C9 position of terminal alpha-2,8-linked sialic acid

Par Conseil national de recherches du Canada

Auteur	Rechercher : Houliston, R.; Rechercher : Endtz, H.; Rechercher : Yuki, N.; Rechercher : Li, J.; Rechercher : Jarrell, Harold; Rechercher : Koga, M.; Rechercher : Van, Belkum; Rechercher : Karwaski, M.; Rechercher : Wakarchuk, Warren; Rechercher : Gilbert, M.
Format	Texte, Article
Sujet	acids; biosynthesis; Campylobacter jejuni; Canada; enzymes; Escherichia coli; gangliosides; Guillain-Barre Syndrome; identification; lipooligosaccharides; prevalence; residues; sialic acid; specificity; strains; structure; syndrome; transfer
Résumé	We have identified a sialate-O-acetyltransferase in the lipo-oligosaccharide biosynthesis locus of Campylobacter jejuni. Strains possessing this locus are known to produce sialylated outer core structures that mimic host gangliosides, and have been implicated in triggering the onset of Guillain-Barre syndrome. The acetyltransferase, which was cloned and expressed as a fusion construct in Escherichia coli, is soluble and homologous with members of the NodL-LacA-CysE family of O-acetyltransferases. This enzyme catalyzes the transfer of O-acetyl groups onto oligosaccharide-bound sialic acid, with a high specificity for terminal a2,8-linked residues. The modification is directed to C9, and not C7, as is believed to occur more commonly in other organisms. Despite their wide prevalence and importance in both eukaryotes and prokaryotes, this is the first report to describe the characterization of a purified sialate-O-acetyltransferase
Date de publication	2006-02-14
Maison d’édition	American Society for Biochemistry and Molecular Biology
Dans	The Journal of Biological Chemistry 281 : 11480–11486.
Langue	anglais
Publications évaluées par des pairs	Oui
Numéro du CNRC	HOULISTON2006
Numéro NPARC	9379851
Exporter la notice	Exporter en format RIS
Signaler une correction	Signaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement	8f171bae-f791-4619-886b-f808c328e570
Enregistrement créé	2009-07-10
Enregistrement modifié	2022-11-18

Date de modification :: 2024-04-20