| DOI | Trouver le DOI : https://doi.org/10.1039/c2an35128e |
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| Auteur | Rechercher : Zheng, D.; Rechercher : Vashist, S.K.; Rechercher : Al-Rubeaan, K.; Rechercher : Luong, J.H.T.1; Rechercher : Sheu, F.-S. |
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| Affiliation | - Conseil national de recherches du Canada. Institut de recherche en biotechnologie du CNRC
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| Format | Texte, Article |
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| Sujet | carbon; glass; glucose; glucose oxidase; immobilized enzyme; Aspergillus niger; chemistry; electrochemistry; electrode; enzymology; genetic procedures; metabolism; methodology; time; Aspergillus niger; Biosensing Techniques; Carbon; Electrochemistry; Electrodes; Enzymes, Immobilized; Glass; Glucose; Glucose Oxidase; Time Factors |
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| Résumé | A rapid and simple procedure was developed for the preparation of a highly stable and leach-proof glucose oxidase (GOx)-bound glassy carbon electrode (GCE). Crosslinked GOx via glutaraldehyde was drop-cast on a KOH-pretreated GCE followed by drop-casting of 3-aminopropyltriethoxysilane (APTES) to form a stable bioactive layer. At -0.45 V, the biosensor exhibited a wide dynamic detection range of 0.5-48 mM for commercial glucose and 1.3-28.2 mM for Sugar-Chex blood glucose linearity standards. Several endogenous electroactive substances and drug metabolites commonly found in blood were tested and provoked no signal response. To our knowledge, the developed procedure is the most rapid method for preparing a glucose biosensor. The biosensor suffered no biofouling after 7 days of immersion in Sugar-Chex blood glucose. With excellent production reproducibility, GOx-bound electrodes stored dry at room temperature retained their initial activity after several weeks. © 2012 The Royal Society of Chemistry. |
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| Date de publication | 2012 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 21269213 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 749bbafd-4f02-4b1d-924d-68487e96c7f1 |
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| Enregistrement créé | 2013-12-12 |
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| Enregistrement modifié | 2020-04-21 |
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