Auteur | Rechercher : Moranelli, F.; Rechercher : Barbier, J. R.1; Rechercher : Dove, M. J.1; Rechercher : Mackay, R. M.; Rechercher : Seligy, V. L.; Rechercher : Yaguchi, M.1; Rechercher : Willick, G. E.1 |
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Affiliation | - Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
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Format | Texte, Article |
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Résumé | Three identical clones coding for a partial sequence of the Schizophyllum commune beta-glucosidase were isolated from a cDNA library in lambda gt11, using polyclonal antibody to the enzyme. The identity was confirmed by comparison of the amino-terminus of a peptide from a protease lys-C digest with the sequence inferred from the cDNA sequence. A comparison of the sequence with that reported for a beta-glucosidase from Candida pelliculosa revealed a region in the latter with 43% identity in amino acid sequence. There was also a similarity in the S. commune beta-glucosidase to an active site sequence proposed for a S. commune endoglucanase, suggesting the possibility of a common catalytic mechanism for these two glucolytic enzymes. |
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Date de publication | 1986-06 |
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Dans | |
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Langue | anglais |
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Numéro du CNRC | MORANELLI1986 |
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Numéro NPARC | 9369927 |
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Exporter la notice | Exporter en format RIS |
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Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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Identificateur de l’enregistrement | 6b8550b0-5432-495f-8a4d-de71d1b03b7a |
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Enregistrement créé | 2009-07-10 |
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Enregistrement modifié | 2020-03-17 |
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