| Téléchargement | - Voir le manuscrit accepté : A microspore embryogenesis protocol for Camelina sativa, a multi-use crop (PDF, 1.2 Mio)
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| DOI | Trouver le DOI : https://doi.org/10.1007/s11240-011-9948-0 |
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| Auteur | Rechercher : Ferrie, A. M. R.1; Rechercher : Bethune, T. D.1 |
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| Affiliation | - Conseil national de recherches du Canada. Institut de biotechnologie des plantes du CNRC
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| Format | Texte, Article |
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| Sujet | Camelina sativa; doubled haploidy; haploids; microspore culture; microspore embryogenesis |
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| Résumé | Camelina [Camelina sativa (L.) Crantz], a member of the Brassicaceae family, has a unique oil profile that has potential both for biofuels and as a food crop. It is essential to have a doubled haploidy protocol in order to enhance breeding of this crop for prairie conditions as well as improve the yield and quality characteristics, Microspore-derived embryos have been produced from Camelina sativa. Buds 1 – 3 mm in length were selected for culture. The microspores were isolated and purified in full-strength B5 extraction medium and cultured in NLN medium with 12.5% sucrose and 12.5% polyethylene glycol 4000 (PEG) without glutamine, at a density of 10,000 microspores per mL. Glutamine was added to the cultures 72 h after extraction to give a final concentration of 0.8 g/L. The microspore cultures were maintained at 24°C in the dark. After 28 days embryos were observed and these were regenerated to plants and selfed seed was produced. The highest embryogenic frequency achieved was 38 microspore-derived embryos from 100,000 microspores. |
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| Date de publication | 2011-04-13 |
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| Dans | |
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| Note | Erratum published in Volume 107, Issue 2, page 371, November 2011. DOI: 10.1007/s11240-011-0025-5 |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | NRCC 50179 |
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| Numéro NPARC | 19366509 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 6b417a6d-d6fd-475e-97f2-6480c375f7f2 |
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| Enregistrement créé | 2012-04-02 |
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| Enregistrement modifié | 2020-04-21 |
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