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Properties and potential advantages of β-galactosidase from Bacteroides polypragmatus

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1007/BF00250030
AuteurRechercher : 1; Rechercher : 1; Rechercher : 2
Affiliation
  1. Conseil national de recherches du Canada
  2. Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
FormatTexte, Article
Résumé
A β-galactosidase (EC 3.2.1.23) from the mesophilic obligate anaerobe, Bacteroides polypragmatus, was purified 172 fold by p-aminophenyl-β-D-thiogalactopyranoside agarose affinity chromatography followed by Bio-Gel P300 chromatography. The presence of Mg2+ and a reducing agent such as dithiothreitol (DTT) or mercaptoethanol was required for enzyme activity. The optimum pH and temperature, as determined from hydrolysis of the substrate analogue o-nitrophenyl-β-D-galactopyranoside (ONPG), for enzyme activity were 6.8 and 45°C, respectively. There was negligible activity loss during incubation at 35°C for up to 13 h. The Km values obtained with ONPG and lactose as substrates were 0.43 mM and 9.09 mM respectively. The enzyme obtained by affinity chromatography was shown to hydrolyze the lactose component of cheese whey; the amount of lactose hydrolyzed was 32% of that expected with pure lactose as the substrate in buffer containing Mg2+ and DTT.
Date de publication
Dans
  • Applied Microbiology and Biotechnology 22, nº 2 (1985) : 114–120.
Langueanglais
Numéro du CNRCPATEL1985
NRCC 24295
Numéro NPARC9370494
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Identificateur de l’enregistrement6a05ec37-f7b5-49cd-ac35-5579aae202e4
Enregistrement créé2009-07-10
Enregistrement modifié2020-03-17
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