| Téléchargement | - Voir le manuscrit accepté : 13C metabolic flux analysis shows that resistin impairs the metabolic response to insulin in L6E9 myotubes (PDF, 735 Kio)
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| DOI | Trouver le DOI : https://doi.org/10.1186/s12918-014-0109-z |
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| Auteur | Rechercher : Guzmán, Shirley; Rechercher : Marin, Silvia; Rechercher : Miranda, Anibal; Rechercher : Selivanov, Vitaly A.; Rechercher : Centelles, Josep J.; Rechercher : Harmancey, Romain; Rechercher : Smih, Fatima; Rechercher : Turkieh, Annie; Rechercher : Durocher, Yves1; Rechercher : Zorzano, Antonio; Rechercher : Rouet, Philippe; Rechercher : Cascante, Marta |
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| Affiliation | - Conseil national de recherches du Canada. Thérapeutique en santé humaine
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| Format | Texte, Article |
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| Sujet | Resistin; Insulin resistance; Glucose metabolism; Fluxomics; Bioinformatics; Tracer-based metabolomics |
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| Résumé | Background: It has been suggested that the adipokine resistin links obesity and insulin resistance, although how resistin acts on muscle metabolism is controversial. We aimed to quantitatively analyse the effects of resistin on the glucose metabolic flux profile and on insulin response in L6E9 myotubes at the metabolic level using a tracer-based metabolomic approach and our in-house developed software, Isodyn.Results: Resistin significantly increased glucose uptake and glycolysis, altering pyruvate utilisation by the cell. In the presence of resistin, insulin only slightly increased glucose uptake and glycolysis, and did not alter the flux profile around pyruvate induced by resistin. Resistin prevented the increase in gene expression in pyruvate dehydrogenase-E1 and the sharp decrease in gene expression in cytosolic phosphoenolpyruvate carboxykinase-1 induced by insulin.Conclusions: These data suggest that resistin impairs the metabolic activation of insulin. This impairment cannot be explained by the activity of a single enzyme, but instead due to reorganisation of the whole metabolic flux distribution. |
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| Date de publication | 2014-09-14 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 21272845 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 5ed7c525-8610-4de2-98b8-b6fcc8e143d3 |
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| Enregistrement créé | 2014-12-03 |
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| Enregistrement modifié | 2020-06-04 |
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