Fluorescence microscopy as a tool for in situ life dete

Par Conseil national de recherches du Canada

DOI	Trouver le DOI : https://doi.org/10.1089/ast.2007.0043
Auteur	Rechercher : Nadeau, J. L.; Rechercher : Perreault, N. N.; Rechercher : Niederberger, T. D.; Rechercher : Whyte, L. G.; Rechercher : Sun, H. J.; Rechercher : Leon, R.
Format	Texte, Article
Résumé	The identification of extant and, in some cases, extinct bacterial life is most convincingly and efficiently performed with modern high-resolution microscopy. Epifluorescence microscopy of microbial autofluorescence or in conjunction with fluorescent dyes is among the most useful of these techniques. We explored fluorescent labeling and imaging of bacteria in rock and soil in the context of in situ life detection for planetary exploration. The goals were two-fold: to target non-Earth-centric biosignatures with the greatest possible sensitivity and to develop labeling procedures amenable to robotic implementation with technologies that are currently space qualified. A wide panel of commercially available dyes that target specific biosignature molecules was screened, and those with desirable properties (i.e., minimal binding to minerals, strong autofluorescence contrast, no need for wash steps) were identified. We also explored the potential of semiconductor quantum dots (QDs) as bacterial and space probes. A specific instrument for space implementation is suggested and discussed.
Date de publication	2008-08
Maison d’édition	Mary Ann Liebert
Dans	Astrobiology 8, nº 4 : 859–874.
Langue	anglais
Publications évaluées par des pairs	Oui
Publication du CNRC	Cette publication n’est pas du CNRC Ce sont des publications qui ont été rédigées par un auteur du CNRC, mais avant que celui-ci soit employé du CNRC.
Numéro NPARC	23001883
Exporter la notice	Exporter en format RIS
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Identificateur de l’enregistrement	57ee6992-5b84-4773-9a65-d7f71dd599df
Enregistrement créé	2017-05-05
Enregistrement modifié	2020-04-15

Date de modification :: 2024-04-19