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DOI | Trouver le DOI : https://doi.org/10.1371/journal.pone.0027559 |
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Auteur | Rechercher : Bhomkar, Prasanna1; Rechercher : Materi, Wayne1; Rechercher : Wishart, David S.1 |
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Affiliation | - Conseil national de recherches du Canada. Technologies de sécurité et de rupture
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Format | Texte, Article |
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Sujet | nanoengineering; cytology; genetic engineering; nanorecorder; reporter gene; synthetic biology; chemical recording devices; Escherichia coli |
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Résumé | Synthetic biology is an emerging branch of molecular biology that uses synthetic genetic constructs to create man-made cells or organisms that are capable of performing novel and/or useful applications. Using a synthetic chemically sensitive genetic toggle switch to activate appropriate fluorescent protein indicators (GFP, RFP) and a cell division inhibitor (minC), we have created a novel E. coli strain that can be used as a highly specific, yet simple and inexpensive chemical recording device. This biological "nanorecorder" can be used to determine both the type and the time at which a brief chemical exposure event has occurred. In particular, we show that the short-term exposure (15-30 min) of cells harboring this synthetic genetic circuit to small molecule signals (anhydrotetracycline or IPTG) triggered long-term and uniform cell elongation, with cell length being directly proportional to the time elapsed following a brief chemical exposure. This work demonstrates that facile modification of an existing genetic toggle switch can be exploited to generate a robust, biologically-based "nanorecorder" that could potentially be adapted to detect, respond and record a wide range of chemical stimuli that may vary over time and space. © 2011 Bhomkar et al. |
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Date de publication | 2011-11-23 |
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Maison d’édition | PLoS |
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Dans | |
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Complété par | Voir les objets (6) |
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Langue | anglais |
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Publications évaluées par des pairs | Oui |
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Numéro NPARC | 21272043 |
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Exporter la notice | Exporter en format RIS |
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Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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Identificateur de l’enregistrement | 57c3872d-b178-4d6e-bfb5-9b3c5e4513bb |
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Enregistrement créé | 2014-05-23 |
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Enregistrement modifié | 2023-07-04 |
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