| Téléchargement | - Voir le manuscrit accepté : Preparation of well-dispersed gold/magnetite nanoparticles embedded on cellulose nanocrystals for efficient immobilization of Papain enzyme (PDF, 1.4 Mio)
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| DOI | Trouver le DOI : https://doi.org/10.1021/am4007534 |
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| Auteur | Rechercher : Mahmoud, Khaled A.1; Rechercher : Lam, Edmond1; Rechercher : Hrapovic, Sabahudin1; Rechercher : Luong, John H. T.1 |
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| Affiliation | - Conseil national de recherches du Canada. Développement des cultures et des ressources aquatiques
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| Format | Texte, Article |
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| Sujet | electrochemical detection; enzyme immobilization; gold nanoparticles; magnetite nanoparticles; papain |
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| Résumé | A nanocomposite consisting of magnetite nanoparticles (Fe3O4NPs) and Au nanoparticles (AuNPs) embedded on cellulose nanocrystals (CNCs) was used as a magnetic support for the covalent conjugation of papain and facilitated recovery of this immobilized enzyme. Fe3O4NPs (10–20 nm in diameter) and AuNPs (3–7 nm in diameter) were stable and well-dispersed on the CNC surface. Energy-dispersive spectroscopy, X-ray diffraction, and Fourier transform infrared spectroscopy were used to evaluate the surface composition and structure of CNC/Fe₃O₄NPs/AuNPs. The nanocomposite was successfully used for the immobilization and separation of papain from the reaction mixture. The optimal enzyme loading was 186 mg protein/g CNC/Fe₃O₄NPs/AuNPs, significantly higher than the value reported in the literature. The activity of immobilized papain was studied by electrochemical detection of its specific binding to the Thc-Fca-Gly-Gly-Tyr-Arg inhibitory sequence bound to an Au electrode. The immobilized enzyme retained 95% of its initial activity after 35 days of storage at 4 °C, compared to 41% for its free form counterpart. |
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| Date de publication | 2013-05-15 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | NRCC 55487 |
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| Numéro NPARC | 21268601 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 4b633fe5-11b4-4570-8b2f-f409be7b5436 |
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| Enregistrement créé | 2013-10-25 |
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| Enregistrement modifié | 2020-06-04 |
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