| DOI | Trouver le DOI : https://doi.org/10.1016/j.febslet.2012.12.005 |
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| Auteur | Rechercher : He, Chuan; Rechercher : Gu, Quanrong; Rechercher : Huang, Min; Rechercher : Xing, James; Rechercher : Chen, Jie1 |
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| Affiliation | - Conseil national de recherches du Canada. Technologies de sécurité et de rupture
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| Format | Texte, Article |
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| Sujet | cell membrane; cell wall; canola cell; cell viability; cell survival; cytological techniques; drug delivery systems; fluorescein isothiocyanate dextran; fluorescein-5-isothiocyanate; centrifugation; microbubble; protoplast |
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| Résumé | We have successfully delivered FITC and FITC-Dextran (70, 250 kDa) into canola protoplasts by centrifuging cells with different amounts of microbubbles at variable centrifuge speed. The efficiency is around 90%, while cell viability remains high. Confocal microscopy images show that both FITC and FITC-Dextran are scattered inside the cytoplasm and the cell nucleus. Pores are observed on canola protoplast cell membranes and cell walls when centrifuged with microbubbles, while the membrane of cells centrifuged alone remain intact and smooth. We hypothesize that the collision between the microbubbles and cells or the bursting of microbubbles are the main reasons for the formation of these pores. Biomaterials can diffuse into the cells once the pathway is created. © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. |
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| Date de publication | 2012-12-19 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 21271799 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 433c6466-34b6-4a4a-a97a-6acb3e9827b2 |
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| Enregistrement créé | 2014-04-22 |
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| Enregistrement modifié | 2020-04-21 |
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