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Haemoglobin-induced oxidative stress is associated with both endogenous peroxidase activity and H 2O 2 generation from polyunsaturated fatty acids

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.3109/10715762.2010.532492
AuteurRechercher : 1; Rechercher : ; Rechercher : ; Rechercher : ; Rechercher : ; Rechercher : ; Rechercher : ; Rechercher :
Affiliation
  1. Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
FormatTexte, Article
Sujetcatalase; hemoglobin; hydrogen peroxide; intercellular adhesion molecule 1; linoleic acid; low density lipoprotein; peroxidase; polyunsaturated fatty acid; probucol; reactive oxygen metabolite; vascular cell adhesion molecule 1; atherogenesis; cardiovascular disease; cell membrane; human cell; incidence; lipid peroxidation; mortality; oxidative stress; protein expression; Anemia, Hemolytic; Antioxidants; Catalase; Endothelial Cells; Fatty Acids, Unsaturated; Hemoglobins; Hydrogen Peroxide; Intercellular Adhesion Molecule-1; Linoleic Acid; Lipoproteins, LDL; Membrane Lipids; Oxidation-Reduction; Oxidative Stress; Peroxidase; Probucol; Reactive Oxygen Species; Sodium Azide; Vascular Cell Adhesion Molecule-1
Résumé
Patients with increased haemolytic haemoglobin (Hb) have 10-20-times greater incidence of cardiovascular mortality. The objective of this study was to evaluate the role of Hb peroxidase activity in LDL oxidation. The role of Hb in lipid peroxidation, H 2O 2 generation and intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) was assessed using NaN3, a peroxidase inhibitor, catalase, a H 2O 2 decomposing enzyme and human umbilical vein endothelial cells (HUVECs), respectively. Hb induced H 2O 2 production by reacting with LDL, linoleate and cell membrane lipid extracts. Hb-induced LDL oxidation was inhibited by NaN3 and catalase. Furthermore, Hb stimulated ICAM-1 and VCAM-1 expression, which was inhibited by the antioxidant, probucol. Thus, the present study suggests that the peroxidase activity of Hb produces atherogenic, oxidized LDL and oxidized polyunsaturated fatty acids (PUFAs) in the cell membrane and reactive oxygen species (ROS) formation mediated Hb-induced ICAM-1 and VCAM-1 expression. © 2011 Informa UK, Ltd.
Date de publication
Dans
  • Free Radical Research 45, nº 3 (2011) : 303–316.
Langueanglais
Publications évaluées par des pairsOui
Numéro NPARC21271470
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Identificateur de l’enregistrement3b78ae26-0248-480c-b8bd-6249954c274e
Enregistrement créé2014-03-24
Enregistrement modifié2020-04-21
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