DOI | Trouver le DOI : https://doi.org/10.1007/978-1-61779-968-6-18 |
---|
Auteur | Rechercher : Agrawal, V.1; Rechercher : Slivac, I.1; Rechercher : Perret, S.1; Rechercher : Bisson, L.1; Rechercher : St-Laurent, G.1; Rechercher : Murad, Y.2; Rechercher : Zhang, J.2; Rechercher : Durocher, Y.1 |
---|
Affiliation | - Conseil national de recherches du Canada. Institut de recherche en biotechnologie du CNRC
- Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
|
---|
Format | Texte, Article |
---|
Sujet | antibiotic g 418; blasticidin S; chimeric antibody; chimeric heavy chain antibody; hygromycin B; phleomycin; plasmid vector; polyethyleneimine; puromycin; unclassified drug; hybrid protein; immunoglobulin heavy chain; monoclonal antibody; animal cell; antibody production; CHO cell; cloning; culture technique; drug activity; genetic transfection; heavy chain; protein expression; suspension cell culture; toxicity; gene expression; gene order; gene vector; genetics; hamster; isolation and purification; metabolism; Antibodies, Monoclonal; CHO Cells; Cricetinae; Gene Expression; Gene Order; Genetic Vectors; Immunoglobulin Heavy Chains; Recombinant Fusion Proteins; Transfection |
---|
Résumé | Camelid single domain antibodies fused to noncamelid Fc regions, also called chimeric heavy chain antibodies (cHCAb), offer great potential as therapeutic and diagnostic candidates due to their relatively small size (80 kDa) and intact Fc. In this chapter, we describe two approaches, limiting dilution and minipools, for generating nonamplified Chinese hamster ovary cell lines stably expressing cHCAb in suspension and serum-free cultures using a stringent antibiotic selection. Neither of the protocols necessitates the acquisition or implementation of expensive automated infrastructures and thus could be applied in any lab with minimal cell culture setup. The given protocol allows the isolation of stable clones capable of generating up to 100 mg/L of antibody in batch mode performed in shaker flasks. © 2012 Springer Science+Business Media, LLC. |
---|
Date de publication | 2012 |
---|
Dans | |
---|
Langue | anglais |
---|
Publications évaluées par des pairs | Oui |
---|
Numéro NPARC | 21269570 |
---|
Exporter la notice | Exporter en format RIS |
---|
Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
---|
Identificateur de l’enregistrement | 3b08d77b-835e-464b-8195-def3eef78cf8 |
---|
Enregistrement créé | 2013-12-12 |
---|
Enregistrement modifié | 2020-04-21 |
---|