DOI | Trouver le DOI : https://doi.org/10.1016/j.chroma.2008.08.112 |
---|
Auteur | Rechercher : Gao, Jin-Ming1; Rechercher : Kamnaing, Pierre1; Rechercher : Kiyota, Taira1; Rechercher : Watchueng, Jean1; Rechercher : Kubo, Takahiro1; Rechercher : Jarussophonb, Suwatchai1; Rechercher : Konishi, Yasuo1 |
---|
Affiliation | - Conseil national de recherches du Canada. Institut de recherche en biotechnologie du CNRC
|
---|
Format | Texte, Article |
---|
Sujet | high-performance displacement chromatography; HPDC; palmatine; dl-Tetrahydropalmatine; protoberberine alkaloid; Enantia chlorantha |
---|
Résumé | Palmatine and its reduced form, dl-tetrahydropalmatine are a group of isoquinoline alkaloids that have been reported to display a variety of biological and pharmacological activities. Both drugs are hydrophilic and are difficult to be purified by conventional purification methods of natural products. A high-performance displacement chromatography (HPDC) method successfully purified palmatine and its semi-synthetic derivative dl-tetrahydropalmatine from crude extract of the African medicinal plant Enantia chlorantha. The crude extract from the root bark of E. chlorantha was fractionated on an analytical reversed-phase C18 column by using 0.1% trifluoroacetic acid (TFA) or acetic acid/H2O as a carrier and cetylpyridinium trifluoroacetate (or acetate) (1.9 mg/mL) in 0.1% TFA (or acetic acid)/H2O as a displacer. Palmatine was quantitatively purified at >98% purity in the fully developed displacement mode. dl-Tetrahydropalmatine was semi-synthesized by NaBH4 reduction from crude palmatine and directly purified by HPDC. Both palmatine and dl-tetrahydropalmatine were identified by high-resolution electrospray tandem mass spectrometry, 1H NMR and 13C NMR. This is the first report of one-step HPDC purification of natural and semi-synthetic products from a complex crude extract. |
---|
Date de publication | 2008-02-05 |
---|
Dans | |
---|
Langue | anglais |
---|
Publications évaluées par des pairs | Oui |
---|
Numéro du CNRC | NRCC 47520 |
---|
Numéro NPARC | 21268255 |
---|
Exporter la notice | Exporter en format RIS |
---|
Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
---|
Identificateur de l’enregistrement | 3859ddb4-cdd2-4365-9648-cf57f4278c7c |
---|
Enregistrement créé | 2013-06-10 |
---|
Enregistrement modifié | 2020-04-15 |
---|