| DOI | Trouver le DOI : https://doi.org/10.1159/000348744 |
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| Auteur | Rechercher : Khan, W.A.; Rechercher : Chisholm, R.1; Rechercher : Tadayyon, S.; Rechercher : Subasinghe, A.; Rechercher : Norton, P.; Rechercher : Samarabandu, J.; Rechercher : Johnston, L.J.1; Rechercher : Knoll, J.H.; Rechercher : Rogan, P.K. |
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| Affiliation | - Conseil national de recherches du Canada. Institut Steacie des sciences moléculaires du CNRC
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| Format | Texte, Article |
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| Sujet | centromere protein B; satellite DNA; atomic force microscopy; centromere; comparative study; DNA sequence; fluorescence microscopy; fluorescent lighting; human cell; human chromosome; metaphase chromosome; protein localization; Cell Line, Tumor; Centromere; Centromere Protein B; Chromatin; Chromosomes, Human, Pair 17; DNA Probes; DNA, Satellite; Fluorescent Antibody Technique; In Situ Hybridization, Fluorescence; Lymphocytes; Metaphase; Microscopy, Atomic Force; Tissue Fixation |
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| Résumé | Despite extensive analyses on the centromere and its associated proteins, detailed studies of centromeric DNA structure have provided limited information about its topography in condensed chromatin. We have developed a method with correlative fluorescence light microscopy and atomic force microscopy that investigates the physical and structural organization of -satellite DNA sequences in the context of its associated protein, CENP-B, on human metaphase chromosome topography. Comparison of centromeric DNA and protein distribution patterns in fixed homologous chromosomes indicates that CENP-B and -satellite DNA are distributed distinctly from one another and relative to observed centromeric ridge topography. Our approach facilitates correlated studies of multiple chromatin components comprising higher-order structures of human metaphase chromosomes. © 2013 S. Karger AG, Basel. |
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| Date de publication | 2013 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 21269955 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 36ff577c-e59c-4f70-b977-cbfca7751a4a |
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| Enregistrement créé | 2013-12-13 |
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| Enregistrement modifié | 2020-10-02 |
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