Global small-angle X-ray scattering data analysis for multilamellar vesicles: the evolution of the scattering density profile model

Par Conseil national de recherches du Canada

DOI	Trouver le DOI : https://doi.org/10.1107/S1600576713029798
Auteur	Rechercher : Heftberger, P.; Rechercher : Kollmitzer, B.; Rechercher : Heberle, F.A.; Rechercher : Pan, J.; Rechercher : Rappolt, M.; Rechercher : Amenitsch, H.; Rechercher : Kučerka, N.¹; Rechercher : Katsaras, J.; Rechercher : Pabst, G.
Affiliation	Conseil national de recherches du Canada. Centre canadien de faisceaux de neutrons du CNRC
Format	Texte, Article
Sujet	Experimental errors; Multi-lamellar vesicles; Scattering density; Small angle X-ray scattering; Small-angle x-rays; Structural information; Structural parameter; Unilamellar vesicle; Binary mixtures; Crystalline materials; Deuterium; Genetic algorithms; Scattering; X ray scattering; X rays
Résumé	The highly successful scattering density profile (SDP) model, used to jointly analyze small-angle X-ray and neutron scattering data from unilamellar vesicles, has been adapted for use with data from fully hydrated, liquid crystalline multilamellar vesicles (MLVs). Using a genetic algorithm, this new method is capable of providing high-resolution structural information, as well as determining bilayer elastic bending fluctuations from standalone X-ray data. Structural parameters such as bilayer thickness and area per lipid were determined for a series of saturated and unsaturated lipids, as well as binary mixtures with cholesterol. The results are in good agreement with previously reported SDP data, which used both neutron and X-ray data. The inclusion of deuterated and non-deuterated MLV neutron data in the analysis improved the lipid backbone information but did not improve, within experimental error, the structural data regarding bilayer thickness and area per lipid. © 2014 International Union of Crystallography.
Date de publication	2014
Dans	Journal of Applied Crystallography 47, nº 1 (2014) : 173–180.
Langue	anglais
Publications évaluées par des pairs	Oui
Numéro NPARC	21272205
Exporter la notice	Exporter en format RIS
Signaler une correction	Signaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement	353cf9f6-14f1-42c5-a8c6-8d8186d79347
Enregistrement créé	2014-07-23
Enregistrement modifié	2020-04-22

Date de modification :: 2024-04-19