| DOI | Trouver le DOI : https://doi.org/10.1255/ejms.399 |
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| Auteur | Rechercher : Li, Jianjun; Rechercher : Tremblay, Tammy-Lynn; Rechercher : Thibault, Pierre; Rechercher : Wang, Christa1; Rechercher : Attiya, Said; Rechercher : Harrison, D2 |
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| Affiliation | - Conseil national de recherches du Canada. Institut de technologie de l'information du CNRC
- Conseil national de recherches du Canada
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| Format | Texte, Article |
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| Sujet | microfabricated device; capillary electrophoresis; nanoelectrospray; tandem mass spectrometry; protein digests; Neisseria meningitidis; proteomics |
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| Résumé | An integrated microsystem providing rapid analyses of trace-level tryptic digests for proteomics application is presented. This modular microsystem includes an autosampler and a microfabricated device comprising a sample introduction port and an array of separation channels together with a low dead-volume facility enabling an interface to nanoelectrospray mass spectrometry. Sequential injection and separation of peptide standards and tryptic digests was achieved with a throughput of up to 30 samples per hour with less than 3% sample carryover. Replicate injections of peptide mixtures indicated that reproducibility of migration time was typically better than 2.3% relative standard deviation (RSD), whereas RSD values of 3.7–11.8% were observed on peak height. Mass spectral detection of sub-micromolar protein digests (< 7 fmol per injection) was achieved using a quadrupole/time-of-flight instrument in less than 2 min per sample with peak widths of 1.8–7.0 s. The analytical potential of this integrated device for the identification of gel-isolated proteins from Neisseria meningitidis immunotype L3 has been demonstrated using both peptide mass-fingerprint database searching and on-line tandem mass spectrometry. |
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| Date de publication | 2001-04-01 |
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| Maison d’édition | SAGE |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | LI2001 |
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| Numéro NPARC | 9378471 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 31fc7eea-c35d-404e-9061-f1f8f3e1e94c |
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| Enregistrement créé | 2009-07-10 |
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| Enregistrement modifié | 2020-03-27 |
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