| Téléchargement | - Voir le manuscrit accepté : Bioproduction of lauryl lactone and 4-vinyl guaiacol as value-added chemicals in two-phase biotransformation systems (PDF, 724 Kio)
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| DOI | Trouver le DOI : https://doi.org/10.1007/s00253-009-2026-4 |
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| Auteur | Rechercher : Yang, Jianzhong1; Rechercher : Wang, Shaozhao1; Rechercher : Lorrain, Marie-Josée1; Rechercher : Rho, Denis1; Rechercher : Abokitse, Kofi1; Rechercher : Lau, Peter C. K.1 |
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| Affiliation | - Conseil national de recherches du Canada. Institut de recherche en biotechnologie du CNRC
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| Format | Texte, Article |
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| Sujet | environmental; ferulic acid; Baeyer-Villiger monooxygenase; phenolic acid decarboxylase; two-phase biotransformation; green chemistry |
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| Résumé | Recombinant Escherichia coli whole-cell biocatalysts harboring either a Baeyer–Villiger monooxygenase or ferulic acid decarboxylase were employed in organic-aqueous two-phase bioreactor systems. The feasibility of the bioproduction of water-insoluble products, viz., lauryl lactone from cyclododecanone and 4-vinyl guaiacol from ferulic acid were examined. Using hexadecane as the organic phase, 10∼16 g of lauryl lactone were produced in a 3-l bioreactor that operated in a semicontinuous mode compared to 2.4 g of product in a batch mode. For the decarboxylation of ferulic acid, a new recombinant biocatalyst, ferulic acid decarboxylase derived from Bacillus pumilus, was constructed. Selected solvents as well as other parameters for in situ recovery of vinyl guaiacol were investigated. Up to 13.8 g vinyl guaiacol (purity of 98.4%) were obtained from 25 g of ferulic acid in a 2-l working volume bioreactor by using octane as organic phase. These selected examples highlight the superiority of the two-phase biotransformations systems over the conventional batch mode. |
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| Date de publication | 2009-05-15 |
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| Dans | |
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| Langue | anglais |
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| Numéro du CNRC | NRCC 49965 |
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| Numéro NPARC | 12689551 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 31681221-3004-49de-bc9f-9ec372572dd9 |
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| Enregistrement créé | 2009-10-26 |
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| Enregistrement modifié | 2020-04-16 |
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