| DOI | Trouver le DOI : https://doi.org/10.1166/jnn.2012.6449 |
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| Auteur | Rechercher : Hao, Yuzhi1; Rechercher : Yang, Xiaoyan; Rechercher : Shi, Yongzhong; Rechercher : Xing, James; Rechercher : Marowitch, Janet; Rechercher : Chen, Jianmin; Rechercher : Chen, Jie1 |
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| Affiliation | - Conseil national de recherches du Canada. Technologies de sécurité et de rupture
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| Format | Texte, Article |
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| Sujet | Covalently bonded; Endosomes; FITC Delivery; Fluorescein isothiocyanate; Magnetic force; Magnetic Single-Walled Carbon Nanotubes (SWCNTs); Nuclear membranes; Plant cells; Protoplast of Canola and Carrot; TEM images; Cell membranes; Plant cell culture; Single-walled carbon nanotubes (SWCN) |
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| Résumé | In this paper, fluoresce in isothiocyanate (FITC) was covalently bonded with magnetic single-walled carbon nanotubes (mSWCNTs) that were purified using our previous method. To demonstrate our design, mSWCNT-FITC was delivered into plant cells (canola and carrot cells) driven by external magnetic forces. From FACS results, the FITC delivery efficiency was about 100% for both two canola and carrot protoplasts, which were further confirmed by the confocal and sectional TEM images. Some mSWCNTs were found trapped both inside the endosomes of canola protoplast and outside endosome near the nuclear membrane of carrot protoplast according to the sectional TEM images. All results showed that mSWCNT is a good delivery carrier for biomolecules. |
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| Date de publication | 2012-08 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 21270230 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 2fc29e6c-1675-4e3c-8ad5-9751c6419f2d |
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| Enregistrement créé | 2014-01-14 |
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| Enregistrement modifié | 2020-04-21 |
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