| DOI | Trouver le DOI : https://doi.org/10.1006/bbrc.1997.7936 |
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| Auteur | Rechercher : Gilbert, Rénald; Rechercher : Nalbanoglu, Josephine; Rechercher : Tinsley, Jonathon M.; Rechercher : Massie, Bernard1; Rechercher : Davies, Kay E.; Rechercher : Karpati, George |
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| Affiliation | - Conseil national de recherches du Canada. Institut de recherche en biotechnologie du CNRC
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| Format | Texte, Article |
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| ISSN | 0006-291X |
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| Sujet | pharmaceutical; Adenoviridae; Cytoskeletal Proteins; Gene Therapy; Gene Transfer Techniques; Genetic Vectors; Injections, Intramuscular; Membrane Proteins; Mice; Mice, Inbred mdx; Muscle, Skeletal; Muscular Dystrophy, Animal; Recombinant Proteins; Utrophin |
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| Résumé | Utrophin is a homologue of dystrophin, the protein whose absence is responsible for Duchenne muscular dystrophy (DMD). As a first step toward clarifying if adenovirus (AV)-mediated utrophin transfer is a possible option to treat DMD, we have constructed an AV expressing utrophin (AdCMV-Utr) and studied utrophin expression after intramuscular injection of mdx mice, the mouse DMD model. Overexpression of utrophin by AdCMV-Utr was marked and nontoxic. The recombinant utrophin was distributed homogeneously at the surface of the muscle fibers. Its expression was sufficient to restore the normal histochemical pattern of α-sarcoglycan and β-dystroglycan at this site. these two proteins are members of the dystrophin associated protein complex whose distribution is greatly reduced at the surface of the DMD muscle. These data indicate that AV-mediated utrophin transfer is an efficient way of utrophin upregulation in muscle and has the potential of becoming a treatment for DMD. |
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| Date de publication | 1998-01-06 |
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| Dans | |
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| Note | UI - 98102816 |
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| Langue | anglais |
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| Identificateur | 10054637 |
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| Numéro du CNRC | 41421 |
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| Numéro NPARC | 3539480 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 27bca0ac-7cf4-4fd1-a01c-1f424c0442f4 |
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| Enregistrement créé | 2009-03-01 |
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| Enregistrement modifié | 2020-03-20 |
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