| DOI | Trouver le DOI : https://doi.org/10.1038/ncomms2204 |
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| Auteur | Rechercher : Borgia, Alessandro; Rechercher : Wensley, Beth G.; Rechercher : Soranno, Andrea; Rechercher : Nettels, Daniel; Rechercher : Borgia, Madeleine B.; Rechercher : Hoffmann, Armin1; Rechercher : Pfeil, Shawn H.; Rechercher : Lipman, Everett A.; Rechercher : Clarke, Jane; Rechercher : Schuler, Benjamin |
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| Affiliation | - Conseil national de recherches du Canada. Institut national de nanotechnologie
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| Format | Texte, Article |
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| Résumé | Theory, simulations and experimental results have suggested an important role of internal friction in the kinetics of protein folding. Recent experiments on spectrin domains provided the first evidence for a pronounced contribution of internal friction in proteins that fold on the millisecond timescale. However, it has remained unclear how this contribution is distributed along the reaction and what influence it has on the folding dynamics. Here we use a combination of single-molecule Förster resonance energy transfer, nanosecond fluorescence correlation spectroscopy, microfluidic mixing and denaturant- and viscosity-dependent protein-folding kinetics to probe internal friction in the unfolded state and at the early and late transition states of slow- and fast-folding spectrin domains. We find that the internal friction affecting the folding rates of spectrin domains is highly localized to the early transition state, suggesting an important role of rather specific interactions in the rate-limiting conformational changes. |
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| Date de publication | 2012-11-13 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 21268918 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 1da9f759-09e0-4023-86c7-0456fffa8971 |
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| Enregistrement créé | 2013-11-25 |
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| Enregistrement modifié | 2020-04-21 |
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