| Autre titre | Metabolic interactions between the Lands Cycle and the Kennedy Pathway of Plant Glycerlipid synthesis |
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| DOI | Trouver le DOI : https://doi.org/10.1105/tpc.112.104604 |
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| Auteur | Rechercher : Wang, Liping1; Rechercher : Shen, Wenyun1; Rechercher : Kazachkov, Michael1; Rechercher : Chen, Guanqun; Rechercher : Chen, Qilin2; Rechercher : Carlsson, Anders S.; Rechercher : Stymne, Sten; Rechercher : Weselake, Randall J.; Rechercher : Zou, Jitao1 |
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| Affiliation | - Conseil national de recherches du Canada. Développement des cultures et des ressources aquatiques
- Conseil national de recherches du Canada. Institut de biotechnologie des plantes du CNRC
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| Format | Texte, Article |
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| Résumé | It has been widely accepted that the primary function of the Lands cycle is to provide a route for acyl remodeling to modify fatty acid (FA) composition of phospholipids derived from the Kennedy pathway. Lysophosphatidylcholine acyltransferase (LPCAT) is an evolutionarily conserved key enzyme in the Lands cycle. In this study, we provide direct evidence that the Arabidopsis thaliana LPCATs, LPCAT1 and LPCAT2, participate in the Lands cycle in developing seeds. In spite of a substantially reduced initial rate of nascent FA incorporation into phosphatidylcholine (PC), the PC level in the double mutant lpcat1 lpcat2-2 remained unchanged. LPCAT deficiency triggered a compensatory response of de novo PC synthesis and a concomitant acceleration of PC turnover that were attributable at least in part to PC deacylation. Acyl-CoA profile analysis revealed complicated metabolic alterations rather than merely reduced acyl group shuffling from PC in the mutant. Shifts in FA stereospecific distribution in triacylglycerol of the mutant seed suggested a preferential retention of saturated acyl chains at the stereospecific numbering (sn)-1 position from PC and likely a channeling of lysophosphatidic acid, derived from PC, into the Kennedy pathway. Our study thus illustrates an intricate relationship between the Lands cycle and the Kennedy pathway. |
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| Date de publication | 2012-11-13 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | NRCC 54658 |
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| Numéro NPARC | 21268461 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 0b793346-d947-47c9-998d-ec5857192b44 |
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| Enregistrement créé | 2013-07-24 |
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| Enregistrement modifié | 2020-04-21 |
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