| Author | Search for: Abulrob, A.1; Search for: Lu, Z.2; Search for: Brunette, E.1; Search for: Pulla, D.2; Search for: Stanimirovic, D.1; Search for: Johnston, L. J.2 |
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| Affiliation | - National Research Council of Canada. NRC Institute for Biological Sciences
- National Research Council of Canada. NRC Steacie Institute for Molecular Sciences
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| Format | Text, Article |
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| Subject | confocal microscopy; glycolipid; membrane rafts; near-field scanning optical microscopy |
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| Abstract | The localization of asialo-GM1 in ordered membrane raft domains in HeLa cells has been examined using a combination of membrane fractionation and fluorescence imaging. The glycolipid is enriched in Triton X-100 insoluble membrane fractions that contain high concentrations of cholesterol and caveolin-1 but is also found in detergent soluble membrane fractions. Near-field fluorescence microscopy shows that a fraction of the asialo-GM1 is localized in small nanoscale clusters that have an upper limit for the average diameter of approximately 90 nm and are partially colocalized with caveolae membrane domains. In addition to clusters, a diffuse, non-clustered population of asialo-GM1 is observed and is hypothesized to correspond to glycolipid isolated in detergent soluble membrane fractions. |
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| Publication date | 2008 |
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| In | |
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| Language | English |
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| NRC number | ABULROB2008B |
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| NPARC number | 9381631 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | ffee2abb-a5ea-4847-a530-083d118fb406 |
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| Record created | 2009-07-10 |
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| Record modified | 2020-04-15 |
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