| Download | - View accepted manuscript: Development of patch-clamp chips for mammalian cell applications (PDF, 937 KiB)
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| Author | Search for: Martinez, Dolores1; Search for: Martina, Marzia2; Search for: Kremer, Laura1; Search for: Monette, Robert2; Search for: Comas, Tanya2; Search for: Salim, Danish2; Search for: Py, Christophe1; Search for: Denhoff, Mike W.1; Search for: Mealing, Geoff2 |
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| Affiliation | - National Research Council of Canada. NRC Institute for Microstructural Sciences
- National Research Council of Canada. NRC Institute for Biological Sciences
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| Format | Text, Article |
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| Subject | Mammalian cells; Planar patch-clamp; Neurochip; Whole-cell recordings |
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| Abstract | We have previously described the designs of two planar patch-clamp neurochips and their application to the electrophysiological study of molluscan neurons cultured on-chip. Neuron attachment and growth over apertures on the neurochip surface permitted the acquisition of whole-cell patch-clamp recordings. To broaden the application of these neurochips from molluscan to mammalian neurons, we conducted a study of cell-to-aperture interaction to optimize conditions for these smaller, more fragile cells. For this purpose, we designed a “sieve” chip having multiple apertures on its surface. Random growth of rat cortical neurons resulted in a 32% (n = 324) probability of cell growth over 2 μm diameter apertures; larger diameters resulted in growth through the aperture. Based on these findings, single-aperture neurochips were fabricated having 2 μm diameter aperture and preliminary electrophysiological recordings from cortical cultures at 14 DIV are presented. The implications of this study for the next-generation neurochips are discussed. |
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| Publication date | 2010-11-28 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 17175248 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | fed3f356-458d-4def-88b1-0531e5c6d26b |
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| Record created | 2011-03-26 |
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| Record modified | 2020-04-17 |
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