Assessment of multidrug resistance on cell coculture patterns using scanning electrochemical microscopy

  1. Get@NRC: Assessment of multidrug resistance on cell coculture patterns using scanning electrochemical microscopy (Opens in a new window)
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Journal titleProceedings of the National Academy of Sciences of the United States of America
Pages92499254; # of pages: 6
Subjectferrocene derivative; ferrocenemethanol; multidrug resistance protein 1; ruthenium complex; unclassified drug; article; cancer cell; cell culture; cell structure; controlled study; electrochemistry; female; HeLa cell; human; human cell; kinetics; multidrug resistance; priority journal; protein localization; quantitative analysis; scanning electrochemical microscopy; target cell; uterine cervix cancer; HeLa cells; microelectrode; MRP1; Cell Culture Techniques; Drug Resistance, Multiple; Female; Ferrous Compounds; HeLa Cells; Humans; Microelectrodes; Microscopy, Fluorescence; Microscopy, Scanning Probe; Multidrug Resistance-Associated Proteins
AbstractThe emergence of resistance to multiple unrelated chemotherapeutic drugs impedes the treatment of several cancers. Although the involvement of ATP-binding cassette transporters has long been known, there is no in situ method capable of tracking this transporter- related resistance at the single-cell level without interfering with the cell's environment or metabolism. Here, we demonstrate that scanning electrochemical microscopy (SECM) can quantitatively and noninvasively track multidrug resistance-related protein 1- dependent multidrug resistance in patterned adenocarcinoma cervical cancer cells. Nonresistant human cancer cells and their multidrug resistant variants are arranged in a side-by-side format using a stencil-based patterning scheme, allowing for precise positioning of target cells underneath the SECM sensor. SECM measurements of the patterned cells, performed with ferrocenemethanol and [Ru(NH3)6]3+ serving as electrochemical indicators, are used to establish a kinetic "map" of constant-height SECM scans, free of topography contributions. The concept underlying the work described herein may help evaluate the effectiveness of treatment administration strategies targeting reduced drug efflux.
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AffiliationNational Research Council Canada (NRC-CNRC)
Peer reviewedYes
NPARC number21270567
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Record identifierfcc2b639-e891-4e35-bed1-4f7e76890995
Record created2014-02-17
Record modified2016-05-09
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