Development of a vectored vaccine against Hepatitis E virus

  1. Get@NRC: Development of a vectored vaccine against Hepatitis E virus (Opens in a new window)
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Journal titleVaccine
Pages28082811; # of pages: 4
Subjectbaculovirus vector; capsid protein; hepatitis E vaccine; parvovirus vector; article; baculovirus expression system; cell density; cell viability; controlled study; expression vector; HEK293 cell line; hepatitis E; Hepatitis E virus; human; human cell; MTT assay; nonhuman; priority journal; protein expression; SF9 cell line; virus recombinant; Western blotting
AbstractHepatitis E virus is a non-enveloped ssRNA virus [1] that causes human acute hepatitis through primarily fecal and oral transmission [2]. Currently, no commercial hepatitis E (HEV) vaccine is available. In the absence of an appropriate cell culture system for HEV propagation, HEV pseudocapsids (ORF2 protein) have been produced either in Escherichia coli or in insect cells and they have been shown to protect monkeys against virus challenge and to be effective in the prevention of natural HEV infection of humans.In this work, we propose to develop a novel candidate vaccine against hepatitis E infection using adeno-associated virus (AAV) as a vector expressing the gene of the truncated capsid protein of HEV (aa 112-aa 660). rAAV will be produced in Sf9 cells using the baculovirus expression vector system.For this purpose, construction of recombinant baculoviruses was performed and viral stocks of BacRep, BacCap for serotypes 2, 5 and 6 were prepared in Sf9 cells. The recombinant baculovirus coding for the truncated capsid protein of HEV (BacITRHEVORF2) was also constructed, the virus titer was equal to 5.41×109PFU/mL, at the third passage. Transduction of HEK 293 EBNA cells with rAAV was carried out; the production of HEVORF2 was confirmed by Western blot. Optimization of rAAV production in Sf9 cells is currently ongoing. © 2014 Elsevier Ltd.
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AffiliationNational Research Council Canada (NRC-CNRC); NRC Biotechnology Research Institute (BRI-IRB)
Peer reviewedYes
NPARC number21272149
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Record identifierfb073230-323b-42ae-95ee-0eb8caa4ce12
Record created2014-07-23
Record modified2016-05-09
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