| Download | - View accepted manuscript: In vitro assay for ABA 8'-hydroxylase: implications for improved assays for cytochrome P450 enzymes (PDF, 1.4 MiB)
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| DOI | Resolve DOI: https://doi.org/10.1007/978-1-61779-231-1_8 |
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| Author | Search for: Krochko, Joan E.1; Search for: Cutler, Adrian J.1 |
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| Affiliation | - National Research Council of Canada. NRC Plant Biotechnology Institute
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| Format | Text, Article |
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| Subject | cytochrome P450; in vitro assay; microsomes; (+) -ABA 8'-hydroxylase; abscisic acid; dormancy; germination; phaseic acid |
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| Abstract | In vitro assays for cytochrome P450 enzymes developed from plant-derived microsomal extracts have not been used extensively for the characterization and quantification of enzyme activities in plant tissues. We describe here an in vitro assay for abscisic acid (ABA) 8 -hydroxylase that was developed using microsomes extracted from ( )-ABA-induced corn suspension cultures. This assay may be useful for further characterization and monitoring of ABA 8 -hydroxylase activities in germinating seeds, seedlings, and other tissues. Additionally, the optimization protocols provided here may be adapted towards improving in vitro enzyme assays for other cytochrome P450 enzymes expressed in plants. |
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| Publication date | 2011-10-02 |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRCC 50161 |
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| NPARC number | 18906336 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | fac896bf-c28c-4104-8b1e-365818d74faf |
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| Record created | 2012-04-01 |
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| Record modified | 2020-04-21 |
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