Production and formulation of adenovirus vectors

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Journal titleAdvances in Biochemical Engineering/Biotechnology
Pages193260; # of pages: 68
SubjectAdenoviridae; Adenovirus; Analytical characterization; bio; cells; cell culture; cell line; cell lines; deficiency; fermentation; formulation; genetic vectors; genetics; gene therapy; protein; purification; therapy
AbstractAdenovirus vectors have attracted considerable interest over the past decade, with ongoing clinical development programs for applications ranging from replacement therapy for protein deficiencies to cancer therapeutics to prophylactic vaccines. Consequently, considerable product, process, analytical, and formulation development has been undertaken to support these programs. For example, "gutless" vectors have been developed in order to improve gene transfer capacity and durability of expression; new cell lines have been developed to minimize recombination events; production conditions have been optimized to improve volumetric productivities; analytical techniques and scaleable purification processes have advanced towards the goal of purified adenovirus becoming a "well-characterized biological"; and liquid formulations have been developed which maintain virus infectivity at 2-8°C for over 18 months. These and other advances in the production of adenovirus vectors are discussed in detail in this review. In addition, the needs for the next decade are highlighted.
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AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedNo
NRC number47790
NPARC number3539701
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Record identifierf98b250d-4a57-4bee-a6e4-3a3212cbbe4f
Record created2009-03-01
Record modified2016-05-09
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