Amino acid sequence and spectroscopic studies of Dutch elm disease toxin, Cerato-ulmin

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EditorSearch for: Sticklan, M.; Search for: Sherald, J.
TypeBook Chapter
Book titleDutch Elm Disease Research: Cellular and Molecular Approaches
Pages152170; # of pages: 19
AbstractThe amino acid sequence of cerato-ulmin (Stevenson et al., 1979), a toxin produced by Ophiostoma (Ceratocystis) ulmi (Buisman) Nannf., the causal agent of Dutch elm disease, has been completely determined. Cerato-ulmin consists of two regions (residues 1–38 and 39–75), and each contains two disulfide bonds. The molecular weight calculated from the amino acid sequence is 7618.7 and the molecular weight determined by Ion-Spray Mass Spectrometetry using a Sciex API III mass spectrometer is reported to be 7618.4 (Feng et al. 1991). The infrared and Raman spectra show that the protein contains about 15% alpha-helix and about 50% beta-sheet. The Raman spectra also revealed no evidence for an S-H stretching vibration near 2580 cm-1 but an intense S-S stretching mode is apparent at 514 cm-1. Thus all eight cysteine residues are linked in disulfide bonds.
Publication date
AffiliationNRC Institute for Biological Sciences; National Research Council Canada; NRC Biotechnology Research Institute; NRC Steacie Institute for Molecular Sciences
Peer reviewedNo
NRC numberYAGUCHI1993
NPARC number9385688
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Record identifierf80c6e99-9764-4df5-9596-de4c33230bc0
Record created2009-07-10
Record modified2016-05-09
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