The capsular polysaccharide of Bacteroides fragilis comprises two ionically linked polysaccharides: J.Biol.Chem.

  1. Get@NRC: The capsular polysaccharide of Bacteroides fragilis comprises two ionically linked polysaccharides: J.Biol.Chem. (Opens in a new window)
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Journal titleJournal Of Biological Chemistry
Pages1823018235; # of pages: 6
SubjectACID; Antibodies; antibody; bacterial; Bacteroides fragilis; BACTEROIDES-FRAGILIS; Biochemistry; biosynthesis; capsular polysaccharide; capsule; Carbohydrate Conformation; Carbohydrate Sequence; cell; CELL-SURFACE; chemistry; complex; COMPLEXES; electron; Form; FORMS; HIGH-RESOLUTION; Immunoelectrophoresis; interaction; Isoelectric Focusing; isolation & purification; Laboratories; Magnetic Resonance Spectroscopy; metabolism; Methods; Microscopy; Microscopy,Immunoelectron; MOLECULAR; Molecular Sequence Data; Molecular Structure; Molecular Weight; MONOCLONAL-ANTIBODIES; MONOCLONAL-ANTIBODY; NMR; NMR spectroscopy; NMR-spectroscopy; POLYSACCHARIDE; Polysaccharides; Polysaccharides,Bacterial; REPEATING UNIT; RESOLUTION; Resolution Nmr Spectroscopy; SPECTROSCOPY; STRUCTURAL; structure; SUBSTITUENT; Support,Non-U.S.Gov't; Support,U.S.Gov't,P.H.S.; surface; ultrastructure; UNIT
AbstractRecently, we have shown that the capsular polysaccharide of Bacteroides fragilis NCTC 9343 is composed of an aggregate of two discrete large molecular weight polysaccharides (designated polysaccharides A and B). Following disaggregation of this capsular complex by very mild acid treatment, high resolution NMR spectroscopy demonstrated that polysaccharides A and B consist of highly charged repeating unit structures with unusual substituent groups (Baumann, H., Tzianabos, A. O., Brisson, J.-R., Kasper, D.L., and Jennings, H.J. (1992) Biochemistry 31, 4081-4089). Presently, we report that the capsular polysaccharide of B. fragilis represents a complex structure that is formed as a result of ionic interactions between polysaccharides A and B. Electron microscopy of immunogold-labeled organisms (with monoclonal antibodies specific for polysaccharides A and B) demonstrated that the two polysaccharides are co-expressed on the cell surface of B. fragilis. We have shown that the purified capsule complex is made up exclusively of polysaccharide A and polysaccharide B (no other macromolecular structure was detected) in a 1:3.3 ratio and that disaggregation of this complex into the native forms of the constituent polysaccharides could be accomplished by preparative isoelectric focusing. Structural analyses of the native polysaccharides A and B showed that they possessed the same repeating unit structures as the respective acid-derived polysaccharides. The ionic nature of the linkage between polysaccharides A and B was demonstrated by reassociation of the native polysaccharides to form an aggregated polymer comparable to the original complex. The distinctive composition of this macromolecule may provide a rationale for the unusual biologic properties associated with the B. fragilis capsular polysaccharide
Publication date
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NPARC number9366371
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Record identifierf0d1d899-00f0-4ab5-9958-a13b128e3eb6
Record created2009-07-10
Record modified2016-05-09
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